What are the methods of gene synthesis?

How can artificial intelligence help codon optimization?

Site-directed Mutagenesis ad its applications

The development history of artificial gene synthesis

How to perform quality control for synthetic genes?

What effect does the endotoxin level of the plasmid have on the experiment?

What are the different levels of plasmid preparation?

What is the definition of PCR cloning? What are its applications?

What is the definition of subcloning? What are its applications?

Difficulties in Long Gene Synthesis

What kind of gene sequence would be difficult to synthesize?

The general process of gene synthesis

Why codon optimization matters?

Codon Usage Bias and Codon Optimization Codon Usage Bias

Gene Synthesis vs. Molecular Cloning

What is the definition of gene synthesis? What are its applications?

Tips for multiplex PCR primer design

Tips for NGS capture probe design

Tips for qPCR probe design

Tips for primer design

What are the commonly used fluorescent modification groups?

What are the functions of Locked Nucleic Acid (LNA) modification?

What are the common types of oligonucleotide base modifications?

What are the functions of Phosphorylation modification?

What are the functions of Digoxigenin modification?

What are the functions of biotin modification?

What are the functions of amino modification?

What are the functions of Spacer modification?

What are the commonly used Oligo purification methods and which applications are they suitable for?

What are the applications of Oligo in quantitative polymerase chain reaction (qPCR)?

What are the applications of Oligo in next-generation sequencing (NGS)?

What parameters can be adjusted and optimized in transient expression?

What is the difference between transient expression and stable expression, and what are their applications?

What are the commonly used cleavage sites in mammalian expression systems?

What are the commonly used tags in mammalian expression systems?

What types of mammalian cell lines are commonly used in expression systems, and what are their characteristics?

What tags are commonly used in insect expression systems to enhance solubility and purification of recombinant proteins?

What insect cells are commonly used for protein expression?

What are the characteristics of an insect expression system?

What yeast strains are commonly used for protein expression?

What are the characteristics of the yeast expression system?

What parameters can be optimized in an E. coli expression system?

How can inclusion bodies be refolded?

What are the causes of inclusion body formation?

Can prokaryotic expression systems be used for expressing eukaryotic proteins?

What are the characteristics of the E. coli expression system?

What are the main applications of cell-free protein expression systems?

What are the main types of cell-free protein expression systems?

What is a cell-free protein expression system?

What are the differences in protein purity requirements for different experiments?

How to Obtain Tag-Free Proteins

Commonly Used Purification Tags

Protein Purification Methods

How to Choose an Appropriate Signal Peptide

Do All Proteins Require Signal Peptides for Expression?

How Does the Molecular Weight of a Protein Affect Its Expression?

How to Choose the Appropriate Recombinant Protein Expression System

Process of Recombinant Protein Production

Why is it necessary to obtain the target protein through recombinant protein expression?