The digoxigenin modification involves attaching digoxigenin molecules to oligonucleotides. Digoxigenin is a naturally occurring plant compound with strong affinity, capable of forming stable complexes with anti-digoxigenin antibodies. This modification finds several applications in molecular biology and biochemistry, with the following functions:

1. Probe labeling: Digoxigenin-modified oligonucleotides serve as molecular probes for detecting the presence and localization of target molecules in various detection and analysis techniques. By incorporating digoxigenin labels into oligonucleotide sequences, target molecules can be detected through binding with anti-digoxigenin antibodies.
2. In situ hybridization: Digoxigenin-modified oligonucleotides are commonly used in in situ hybridization experiments to detect and localize specific nucleic acid sequences in cellular or tissue samples. Visualizing target nucleic acid sequences is achieved through binding with anti-digoxigenin antibodies.
3. Immunoassays: Digoxigenin-modified oligonucleotides find utility in various immunoassay techniques, such as enzyme-linked immunosorbent assays (ELISA) or Western blotting. Binding with anti-digoxigenin antibodies enables quantitative detection and analysis of target molecules.
4. Drug delivery and targeting: Digoxigenin-modified oligonucleotides can also be used for drug delivery and targeted therapy. Incorporating digoxigenin labels into oligonucleotide drugs enables targeted delivery to specific cell types, thereby enhancing drug efficacy and reducing side effects.
5. DNA probes and primers: Digoxigenin-modified oligonucleotides serve as DNA probes or primers in applications such as polymerase chain reaction (PCR), DNA hybridization, and DNA sequencing. Digoxigenin labeling confers specific functionalities and applications to oligonucleotides in these techniques.

Digoxigenin-modified oligonucleotides have widespread applications in life science research, including molecular diagnostics, gene expression analysis, drug development, etc., providing researchers with a powerful tool for studying and understanding biological processes.

GenCefe provides customized DNA oligo modification/labeling services, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes/primers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade modified primers/probes.