Selecting the appropriate recombinant protein expression system is crucial for the successful production of the target protein. Different expression systems have their own advantages and disadvantages, making them suitable for various applications. Here are the main expression systems and criteria for their selection:

Selection Criteria

1. Protein Properties: Consider if the target protein requires post-translational modifications; if so, choose mammalian or insect cell systems.
2. Expression Yield: For high-yield protein production, bacterial or yeast systems may be more suitable.
3. Purification Complexity: Some systems (e.g., yeast) can secrete proteins into the medium, simplifying purification.
4. Cost and Time: Bacterial systems are low-cost and fast, suitable for rapid experiments; mammalian systems are high-cost and time-consuming, suitable for high-demand applications.
5. End Use: Proteins for drug development and therapeutic use usually require mammalian cell systems to ensure proper post-translational modifications and bioactivity.

By considering these factors, you can select the most appropriate expression system to meet specific research or production needs.

GenCefe provide one-stop solutions from  codon optimization ,  gene synthesis , to  protein expression and purification . In addition to four conventional expression systems of  bacterial ,  yeast ,  insect cell , and  mammalian cell , we have also developed a patented  cell free expression  technology, which is suitable for the production of difficult proteins such as toxic proteins and membrane proteins.