Transient Expression: 

   ●    Definition: Transient expression occurs when introduced genetic material (DNA or RNA) remains episomal (outside the genome) in the cell and is not integrated into the host genome. As a result, the gene is expressed for a short period, typically lasting 1-7 days, depending on the system and cell type. 

   ●    Applications: 

          ●      Rapid Protein Production: Ideal for quick protein production in a short time, often used in research labs for testing protein function, screening, and structural analysis. 

          ●      Preliminary Functional Studies: Used for short-term functional assays, promoter activity studies, and gene regulation experiments. 

          ●      High-Throughput Screening: Useful for high-throughput screening of compounds or genes, as it is faster to implement compared to stable expression. 

Stable Expression: 

   ●    Definition: Stable expression involves the integration of foreign DNA into the host cell genome, leading to continuous and long-term expression of the gene. Cells that integrate the gene are selected using antibiotics or other markers, and the expression is permanent as long as the cell line is maintained. 

   ●    Applications: 

          ●      Therapeutic Protein Production: Widely used in industrial settings for the large-scale production of therapeutic proteins, monoclonal antibodies, and vaccines. 

          ●      Drug Screening and Development: Provides a reliable system for long-term drug testing and development. 

          ●      Functional Studies: Useful in long-term functional studies, gene knockouts, or overexpression studies where sustained expression is required. 

          ●      Cell Line Development: Essential for developing cell lines for biopharmaceutical production or disease modeling. 

Transient expression is used for short-term, rapid studies where quick protein production or gene function testing is required, while stable expression is suitable for long-term studies, therapeutic protein production, and drug development, providing continuous and consistent gene expression over extended periods. 

GenCefe provide one-stop solutions from codon optimization, gene synthesis, to protein expression and purification. In addition to four conventional expression systems of bacterial, yeast, insect cell, and mammalian cell, we have also developed a patented cell free expression technology, which is suitable for the production of difficult proteins such as toxic proteins and membrane proteins.