Why is it necessary to obtain the target protein through recombinant protein expression?
Why is it necessary to obtain the target protein through recombinant protein expression?

Recombinant protein expression is a widely used technique in biology and biotechnology that allows the production of large quantities of target proteins in host cells. Here are the main reasons and advantages of using recombinant protein expression:


  1. Efficient Production: Recombinant protein expression systems enable the rapid production of large amounts of target protein. This is crucial for applications that require substantial quantities of protein for research or manufacturing.
  2. Protein Purity and Consistency: These systems can produce highly pure proteins with consistent structure and function in each production batch. This is essential for scientific research and drug development.
  3. Functional Proteins: Many naturally occurring proteins may lose their activity or function during extraction. Recombinant expression ensures that proteins retain their functionality during production.
  4. Difficult-to-Extract Proteins: Some proteins are present in very low quantities in their natural hosts and are difficult to extract. Recombinant expression allows these proteins to be efficiently expressed in easily cultured and processed host cells (such as bacteria, yeast, or mammalian cells).
  5. Flexibility of Genetic Manipulation: Recombinant techniques enable genetic modifications of proteins, such as adding tags, mutations, or fusion proteins, to facilitate subsequent research and applications.
  6. Safety: Producing certain proteins, such as toxins or pathogen-related proteins, through recombinant expression can be done in a controlled and safe environment, reducing biosafety risks.
  7. Cost-Effectiveness: Recombinant expression systems are often more cost-effective compared to extracting proteins from natural sources, especially for large-scale production.


GenCefe provide one-stop solutions from codon optimization,gene synthesis, to protein expression and purification. In addition to four conventional expression systems of bacterial, yeast, insect cell and mammalian cell, we have also developed a patented cell free expression technology, which is suitable for the production of difficult proteins such as toxic proteins and membrane proteins.

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