GenCefe's DNA oligo platform integrates sequence design, synthesis, modification, purification, and quality control into a streamlined and coordinated workflow. The service supports a broad range of oligonucleotide formats, including standard primers, high-purity oligos, labeled or modified oligos, qPCR primers and probes, FISH probes, STR primers, circular DNA, and PCR assay development applications. 

To meet diverse project needs, GenCefe provides flexible DNA oligo synthesis solutions tailored for research, diagnostic development, and molecular assay workflows. The production and quality control system is designed to ensure high consistency, reliable performance, and efficient delivery across projects. 

Platform Advantages:

· Integrated workflow from sequence design to DNA oligo synthesis, modification, and QC

· Broad coverage for research, diagnostic, and sequencing-related applications

· Flexible customization for different oligonucleotide types and project requirements

· Standardized QC under ISO 9001 and ISO 13485 certified systems

GenCefe is committed to maintaining product quality and project confidentiality throughout DNA oligo synthesis services. Quality control is implemented through standardized production processes, purity verification, and application-specific evaluation to ensure reliable performance and consistent batch quality. 

Quality Control

· Standardized process control from order processing to final delivery

· Purity and quality verification of synthesized oligonucleotides

· Customized QC options available based on project requirements

· Batch-to-batch consistency monitoring for reliable performance

· Documented QC support for research and diagnostic applications

Confidentiality

· Secure handling of customer sequences and project information

· Restricted access to data by authorized personnel only

· No disclosure of project information to third parties without consent

· NDA agreements available upon request

GenCefe's DNA oligo synthesis services provide high-quality oligonucleotides that support molecular biology research, diagnostic assay development, and downstream experimental workflows. The service is designed to ensure reliable performance, flexible customization, and efficient project progression from sequence submission to final delivery.

Value Highlights:

· Consistent oligo quality supported by controlled synthesis and QC systems

· Reliable batch-to-batch reproducibility for experimental accuracy

· Flexible customization for primers, probes, and modified oligonucleotides

· Technical support to improve design and workflow efficiency

· Suitable for research, diagnostic development, and molecular applications

1） 125 nt, Spacer18 modification, multiple Poly-T sequences, purity>95.58%, molecular weight bias<0.05%

2） qPCR Primers & Probes: Comparison of Probe Fluorescence Intensity

Under the same experimental conditions, after 120 minutes of incubation, the probe of GenCefe has a higher fluorescence signal intensity, which is about 1.5 times higher than that of Company SG. This shows that the probe synthesized by GenCefe has higher sensitivity

GenCefe streamlines DNA oligo ordering from sequence submission to final delivery. Customers can submit requests via email or online form, and our scientific team provides guidance based on application requirements.

1. Request a Quote & Submit Sequence: Email the order request with sequence details, including oligo type, length, modifications, and quantity requirements.

2. Sequence Review & Design Confirmation: The sequence is reviewed and the synthesis plan is confirmed according to project specifications.

3. Synthesis & Modification: DNA oligos are synthesized and modified under controlled production workflows.

4. Quality Control & Documentation: Quality and purity checks are performed according to service level and application needs, with related QC data and documentation prepared for delivery.

5. Global Delivery: Final oligonucleotides are shipped with secure packaging and full tracking support.

Get a Quote: [Submit Sequence Now]

GenCefe's DNA oligo synthesis services are built on a streamlined and well-controlled workflow that ensures consistency, reliability, and smooth execution from sequence handling to final delivery. With technical expertise and standardized production systems, we support diverse sequence requirements and application needs across research, diagnostic, and molecular biology workflows. 

By integrating synthesis, modification, and quality control within a unified process, GenCefe helps reduce operational complexity and ensures stable performance across different project types. Supported by ISO 9001 and ISO 13485 certified quality management systems, our services provide traceability, controlled documentation, and dependable execution that customers can rely on for long-term collaboration.

GenCefe's DNA oligo synthesis services support a wide range of molecular biology research and molecular diagnostic applications. The service is designed for both standard experimental workflows and application-specific oligonucleotides requiring high purity or specialized modifications.

Research Applications:

· Standard PCR primers for routine molecular biology workflows

· High-purity primers for sensitive and quantitative applications

· Modified or labeled oligonucleotides for specialized experimental designs

Molecular Diagnostics:

GenCefe provides oligonucleotides for multiple diagnostic assay formats, supporting reliable performance and application flexibility.

1) qPCR-based Applications:

· qPCR primers and probes for assay development

· IVD-related qPCR primers and probes for diagnostic workflows

2) Forensic Analysis:

STR fluorescent-labeled primers for forensic identification and analysis

3) Other Assay Development:

· FISH probes for spatial detection studies

· Circular DNA-related oligonucleotides

· PCR assay design and development support

GenCefe offers standard primers, high-purity primers, modified/labeled oligos, qPCR primers and probes, FISH probes, STR primers, and circular DNA-related oligonucleotides.

Yes. We support a wide range of customized modifications for primers and probes based on project requirements. For example, 5'/3' phosphorylation, fluorescent labels (e.g., FAM, Cy5), biotin, LNA, methylation, and more.

For a full list of available oligo modifications and fluorescent labeling options, please visit the Oligonucleotide Modifications page. 

GenCefe operates under ISO 9001 and ISO 13485 certified quality systems with standardized production and QC processes.

Our oligonucleotides support molecular biology research, molecular diagnostics, NGS, forensic analysis, FISH, circular DNA studies, and PCR assay development.

Customers can submit oligo sequences via the online form or send requests by email to [email protected].

Standard DNA oligos are typically shipped within 1–3 business days. Modified or RNA oligos usually require 3–7 business days depending on complexity.

Available purification methods include desalting, MOP, HPLC, and PAGE. The appropriate method is recommended based on sequence length and application.