DNA Oligo QC
GenCefe Biotech has established a strict quality control system for oligonucleotide products and has passed ISO9001 & ISO13485 certification. In addition to standard QC items, we also provide comprehensive customized QC items for different applications, such as scientific research and in vitro diagnostics.
Oligo Detection Methods:
| Detection Items (methods) | Equipment | Brief Introduction |
| Appearance | 30x magnifying glass inspection lamp | Detect the presence of undried product, impurities, etc. |
| Quantitative (UV) | UV spectrophotometer | Detect the quantitative of Oligos |
| Molecular weight (MS) | Electron Spray Mass Spectrometry | Detect the molecular weight of Oligos |
| Purity (HPLC) | High performance liquid chromatography | Detect the purity of Oligos |
| Purity (CE) | Capillary electrophoresis | |
| Purity (PAGE) | Vertical gel electrophoresis | |
| CT value(qPCR) | Fluorescent quantitative PCR | Detect the positive target detection capabilities of the qPCR primer & probes |
| Fluorescence value(qPCR) | Detect the fluorescence value of qPCR primer & probes when amplifying positive targets | |
Human genomic DNA contamination (qPCR) | Detection of human genomic DNA contamination in qPCR primer & probes. | |
| NTC amplification (qPCR) | Detect abnormal amplification signals of qPCR primer & probes in a template-free system. | |
| Cross Contamination Rate (NGS) | High throughput sequencer | Detect the probability of cross-contamination between different oligos |
Oligo QC Items:
| Detection Item | Primer Type | Primer | qPCR Primer | qPCR Probe | NGS Primer | Note |
Property detection | Appearance | Dry powder: uniform powder or transparent solid, without obvious impurities | Standard Detection | |||
| Liquid: colorless/specific color liquid, clear and transparent, without obvious impurities | ||||||
Quantitative (UV) | Error rate(％)≤±10％ | |||||
Molecular weight (MS) | Error rate(％)s＋0.05％ | |||||
| Purity (HPLC) | RPC≥85%;MOP≥90%;PAGE≥95%;HPLC/HPLC IVD≥95% | Customiz Detection | ||||
| Purity (CE) | Vary according to the purification method and the oligo length | |||||
| Purity (PAGE) | Clear main product band, correct molecular weight, no obvious impurities | |||||
Function Detection | Ct Value (qPCR) | - | A normal amplification curve can be detected within 45 cycles, and the Ct value is within a reasonable range | Customize Detection | ||
Fluorescence value (qPCR) | - | According to the agreement | ||||
Contamination Detection | Human genomic DNA contamination (qPCR) | - | No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40 | |||
NTC amplification (qPCR) | - | No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40 | ||||
Cross Contamination (NGS) | - | - | - | Cross Contamination Rate(%)≤0.1% | ||
