Codon Optimization
Host-specific codon optimization to support efficient recombinant protein expression.
Host Compatibility
AI-Assisted Design
Proven Results
Integrated Process
Codon optimization adjusts synonymous codon selection according to the selected expression host while maintaining the intended protein sequence. GenCefe’s proprietary optimization algorithm combines AI-assisted design with gene synthesis considerations, balancing host-specific codon preferences and sequence complexity to develop optimized coding sequences that support efficient protein expression while maintaining synthesis feasibility.
Our optimization considers both expression efficiency and synthesis difficulty during sequence design. Optimization results have been evaluated through extensive internal testing and customer projects, with multiple cases demonstrating significant improvements in recombinant protein expression levels. As part of our gene synthesis workflow, codon optimization is integrated with sequence design and synthesis to provide practical, host-adapted gene designs for downstream applications.
Key Features:
Host-Specific Optimization: Tailored to the codon preferences of selected expression hosts.
AI-Assisted Design: Advanced algorithms for optimized coding sequence design.
Expression Performance: Designed to support higher protein expression levels.
Synthesis-Aware Optimization: Balancing expression efficiency with gene synthesis feasibility.
Case Study: 7.4-Fold increase in eukaryotic protein expression in E. coli
Objective : To evaluate the expression level of a eukaryotic gene optimized by our Codon Optimization Tool in E. coli.
Method : The wild-type SKN-1 gene was analyzed and optimized by our codon optimization software and procedure. The optimized gene was then cloned into an E. coli expression vector. The bacteria containing this gene were cultured and protein expression was induced, with a wild-type gene as comparison. The protein expression was checked by Western Blot. Three clones of each optimized or wild-type gene were examined and the result was shown in Figure 1.
Result : The three clones containing optimized SKN-1 gene showed a 6-fold, 7.2-fold, and 9-fold increase separately, with an average of 7.4-fold increase, over the clones containing wild-type.
Figure 1.: Left: Protein electrophoresis of bacteria lysate expressing wild-type and optimized SKN-1 gene. Right: protein quantitation by Western Blot.
Gene Synthesis Services
Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.
