Custom IVT mRNA Synthesis Services
GenCefe Biotech provides custom IVT mRNA synthesis services for research and therapeutic development. From gene synthesis and plasmid preparation to in vitro transcription, purification, and quality control, we offer a streamlined end-to-end workflow tailored to your project requirements.
We support customized coding sequences, UTR designs, poly(A) tail options, and nucleotide modifications to meet diverse application needs, including protein expression, vaccine research, gene editing, and preclinical studies. By integrating the entire workflow on a single platform, we help accelerate your research with reliable quality and efficient delivery.
Service Workflow
Custom synthesis options
5’ Cap | Poly A Tail | Modifications |
Cap0 (m7GpppN) | 110±5 nt A Tail, | Pseudouridine |
Service Specifications
Step | Description | Turnaround Time | Deliverables | Order |
Gene Synthesis | Codon optimization | Starting from 2 weeks | Sanger sequencing result for the synthesized gene | |
Plasmid Preparation | Plasmid preparation | |||
IVT mRNA | 20 μg-2mg | 1-2 weeks | ||
5 mg | 2-3 weeks | |||
>5 mg | ||||
Standard QC items | appearance, mRNA length, sequence accuracy, RNA concentration, A260/280, purity | / | CoA | |
Customized QC items (optional, extra charge) | PolyA tailing efficiency, capping efficiency, template DNA residue, protein residue, RNase residue, dsRNA detection, endotoxin content, bioburden |
* LNP-encapsulated mRNA cannot be lyophilized and can only be shipped in liquid form at low temperatures, which will incur additional dry ice and shipping costs.
Off-the-shelf mRNA Products
To support faster project initiation, GenCefe Biotech also offers a selection of ready-to-order catalog mRNA products covering popular research targets. Below are some of our commonly used mRNAs. For additional products and ordering information, please explore our Catalog mRNA page.
Product Name | Size | Turnaround Time | Order |
eGFP mRNA | 100 μg/tube | 3-5 days | |
mCherry mRNA | 100 μg/tube | 3-5 days | |
Luciferase mRNA | 100 μg/tube | 3-5 days | |
mCherry-eGFP mRNA | 100 μg/tube | 3-5 days | |
Cas9 mRNA | 100 μg/tube | 3-5 days | |
| For additional products and ordering information, please explore our Catalog mRNA page. | |||
