sgRNA Synthesis
sgRNA (single guide RNA) is known as the command center of the CRISPR/Cas9 gene editing system, which can guide Cas9 to perform targeted editing of DNA. Chemically synthesized sgRNAs usually have low cytotoxicity, and their stability can be enhanced by adding a variety of modifications, thereby reducing off-target effects. Compared with in vitro transcribed sgRNA and plasmid sgRNA, it has the advantages of lower cost, higher editing efficiency, and better batch-to-batch consistency.
GenCefe Biotech provides sgRNA design and chemical synthesis services. Deliverables are tested by mass spectrometry for sequence accuracy and purified by HPLC. We are committed to providing you with sgRNAs with low toxicity, batch-to-batch stability, and high editing efficiency.
sgRNA Synthesis Service Specifications
Product Name | Length | Size | Purification | Turnaround Time (days) | Price |
Chemically synthesized sgRNA | 20-150 nt | >2 OD | HPLC | 7 |
sgRNA Libraries:
| Services | Description | Turnaround Time | Order |
| sgRNA Design | gRNA design targeting specific genes according to customer’s requirements | 1-2 weeks | Get a Quote |
| sgRNA Library Construction | sgRNA and control synthesis Constructed the sgRNA to designated vector | 5-7 weeks | |
| Plasmid Preparation | Transfection grade plasmid preparation | 1 week | |
| QC | Extra 20 business days will be needed if QC by NGS is required | Get a Quote |
