qPCR Primers & Probes
Custom qPCR primers and probes with flexible specifications and reliable quality.
Certified Quality
GMP-Like Facility
Reliable Probes
Consistent Quality
Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, enables rapid and sensitive quantification of specific target sequences. Primer and probe quality can influence assay performance and the reliability of results, making sequence accuracy, oligo purity, and appropriate fluorescent labeling important considerations when selecting qPCR oligonucleotides.
GenCefe Biotech provides custom qPCR Primers & Probes through an advanced oligonucleotide synthesis and modification platform, supported by an experienced technical team. With flexible specifications, high-purity options, reliable fluorescent labeling, and standardized quality control, we support research and diagnostic applications with consistent product quality.
Key Features:
Certified Quality Management: Production supported by ISO 9001 and ISO 13485 certified quality management systems.
Contamination Control: GMP-like cleanroom and contamination-control measures help minimize contamination-related NTC interference.
Advanced Synthesis & Labeling: Reliable synthesis, high-purity options, and fluorescent labeling.
Batch-to-Batch Consistency: Standardized procedures and quality control support consistent product quality across batches.
qPCR Primers & Probes Service Specifications:
| Service Type | Purification Method | Length | Deliverables |
| qPCR Probe | HPLC | 11-59 nt | Tube or 96-well plate Dry powder COA |
| qPCR Primer | HPLC | 11-59 nt |
Please download and fill out the oligo order form and send it via email to [email protected]. Please note the minimum order size of custom oligo synthesis is $50/order.
Modification/Labeling/Dual-Labeling: view the full list
| Rare Bases Modifications | Phosphorothioate Modifications | Phosphorylation Modifications |
| Biotin Modification | Amino Modifications | Thiol Modifications |
| Digoxigenin Modifications | Fluorescent Labeling | Quenching Groups |
| Intermediate Modifications | Other modifications | Dual-Labeling |
qPCR Primers & Probes Case Studies:
1.Comparison of Probe Fluorescence Intensity
 | Under the same experimental conditions, after 120 minutes of incubation, the probe of GenCefe has a higher fluorescence signal intensity, which is about 1.5 times higher than that of Company SG. This shows that the probe synthesized by GenCefe has higher sensitivity |
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2.FAM probe multi-component enzyme digestion verification
 | The probes synthesized by GenCefe has a high fluorescence intensity, indicating that the probes have high sensitivity. Compared with the probes synthesized by Company S, the probe synthesized by GenCefe shows better stability. |
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3.TaqMan Probe Comparison
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TaqMan Probe (GenCefe) | Compared with the probes provided by Company SG, the GenCefe probes showed better Ct values and fluorescence intensity, and the detection results in different channels were stable. |
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| Detection result of GenCefe TaqMan Probes in different channels | ||
4.Dual-Quenching Probe
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| Dual-Quenching Probe (Company TF) | Dual Quenching Probe (GenCefe) |
| Compared with similar probes from TF, the GenCefe dual-quenching probe shows better Ct value and fluorescence intensity, with the fluorescence intensity increased by >100%. | |
Oligo Synthesis Services
A detailed introduction to GenCefe’s DNA oligo synthesis capabilities, customization options, and quality assurance for diverse research and molecular diagnostics applications.
