Circular DNA
Custom single-stranded circular DNA synthesis tailored to your research needs.
GenCefe Biotech provides custom Circular DNA synthesis, specializing in circular single-stranded DNA (CssDNA) for gene expression, gene therapy, and vaccine research.
With established CssDNA synthesis technology, GenCefe offers customized synthesis solutions based on customer-provided sequences and project requirements, supporting research applications that require circular single-stranded DNA.
Key Features:
Enhanced Stability: The circular DNA structure helps protect against intracellular degradation and may support sustained expression.
Transfection Performance: CssDNA may offer transfection advantages over linear DNA in certain research applications, including gene expression and DNA vaccine studies.
Versatile Applications: Supports research involving gene expression vectors, virology, nanotechnology, and gene editing.
Custom Synthesis Solutions: Customized CssDNA synthesis based on customer-provided sequences and project-specific requirements.
Service Specifications:
Length | Turnaround Time | Order | Deliverables | |
CssDNA Synthesis | ≤60 nt | 7+ days | Lyophilized CssDNA CoA Report | |
61-100 nt |
Case Study:
Synthesize ~20 nt DNA oligo, and connect the 5' phosphate end of the single-stranded DNA to the 3' hydroxyl end through ligase to form a circular structure.
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Left: LC-MS during the cyclization reaction | Right: LC-MS after the cyclization reaction | |
Fig. 1 The target molecular weight before and after cyclization differs by 18.8, which is consistent with the characteristics of linearized DNA cyclization. During the cyclization of linearized DNA, an additional phosphodiester bond is formed, and a water molecule is removed, resulting in a decrease in molecular weight of 18. | ||
| Fig. 2 Verification of DNA cyclization using linear DNA digestion enzyme. Linear and circular DNA fragments of the same size were treated with linear digestion enzyme. The linear DNA was digested, while the circular DNA showed no significant change. 1: Marker 2: Linear DNA 3: Linear DNA treated with linear digestion enzyme (digested) 4: Circular DNA 5: Circular DNA treated with linear digestion enzyme (undigested) | |
Circular Oligonucleotide Synthesis
Explore GenCefe’s circular oligonucleotide synthesis solutions, including circular ssDNA and circular RNA (circRNA), supported by flexible synthesis technologies for diverse research needs.
Oligo Synthesis Services
A detailed introduction to GenCefe’s DNA oligo synthesis capabilities, customization options, and quality assurance for diverse research and molecular diagnostics applications.
