Subcloning & PCR Cloning
Custom Cloning Solutions for Tailored Plasmid Construction
Subcloning & PCR Cloning services help researchers construct plasmids containing their target genes for downstream molecular biology applications. GenCefe can clone target genes into designated sites within specified vectors, supporting different requirements for vector selection, insert location, and final plasmid construct design.
Our technical team has experience working with more than 100 commercially available vectors and can also develop customized cloning solutions for customer-provided vectors. From gene synthesis and cloning to plasmid preparation, GenCefe provides integrated project support to help researchers obtain the plasmid constructs required for their downstream workflows.
Key Features
Sanger-Verified Accuracy: Final plasmid deliverables are verified by Sanger sequencing to confirm sequence accuracy.
Flexible Vector Cloning: Target genes can be cloned into designated sites within specified vectors according to project requirements.
Broad Vector Experience: Our technical team has experience working with more than 100 commercially available vectors and can develop customized cloning solutions for customer-provided vectors.
Integrated Molecular Biology Support: Connect gene synthesis, subcloning or PCR cloning, and plasmid preparation within an integrated project workflow.
Subcloning Service Specifications
| Service Type | Gene Length | Turnaround Time (Business Day) | Price |
| Subcloning bundled with gene synthesis | <3000 bp | 5 Business Days | $29/gene |
| 3001 bp ~ 5000 bp | 8-10 Business Days | Get a Quote | |
| >5000 bp | Get a Quote | Get a Quote | |
| Subcloning Only | <3000 bp | 6-10 Business Days | $59/gene |
| 3001 bp ~ 5000 bp | 10-12 Business Days | Get a Quote | |
| >5000 bp | Get a Quote | Get a Quote |
PCR Cloning Service Specifications
| Gene Length | Turnaround Time (Business Day) | Price |
| < 1000 bp | 5-8 Business Days | $79/gene |
| 1000 bp ~ 3000 bp | 10-14 Business Days | Get a Quote |
| 3001-5000 bp | 10-14 Business Days | Get a Quote |
| > 5000 bp | Get a Quote | Get a Quote |
Vector Storage Service
For vectors customized through GenCefe and vectors provided to GenCefe for project use, we offer 3 years of free storage. If you require a longer storage period or have specific storage requirements, please contact technical support.
Delivery Specifications
4 μg of lyophilized plasmid
Electronic sequence chromatograms covering the target gene
Electronic plasmid construct map
Quality assurance certificate
Vector List
GenCefe has experience working with commercial vectors for mammalian, bacterial, yeast, and baculovirus/insect expression systems. The list below highlights commonly used vectors that our technical team has experience working with. If you have a specific vector, target gene, or designated cloning site, please contact our technical team to discuss your requirements.
Gene Synthesis Services
Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.
