Dual Approaches
Flexible Lengths
Flexible Options
Cyclization QC
With growing research interest in circular RNA (circRNA) for RNA therapeutics, vaccine development, and sustained protein expression, the demand for flexible circRNA synthesis solutions continues to grow.
GenCefe Biotech offers custom circRNA synthesis through complementary chemical and enzymatic synthesis platforms, supporting different sequence lengths, modification requirements, and purity needs. Our flexible capabilities enable tailored synthesis solutions for diverse research projects.
Key Features:
Dual-Platform Technology: Chemical and enzymatic synthesis platforms support different circRNA length ranges and research requirements.
Short circRNA Synthesis: Chemical synthesis supports circRNA segments of up to 100 nt for applications such as miRNA sponges and functional fragment screening.
Long circRNA Synthesis: Enzymatic synthesis is suited to longer circRNA constructs of 200 nt or more for functional studies and drug development research.
Custom Synthesis Support: GenCefe’s experienced team provides tailored support for circRNA sequence, modification, purity, and production requirements.
Service Specifications:
| CircRNA Synthesis | Length | Turnaround Time | Order | Deliverables |
Chemical Synthesis | ≤60 nt | 7+ days | Lyophilized circular RNA CoA Report | |
61-100 nt | ||||
Enzymatic Synthesis | >200 nt | 2+ weeks |
Case Study:
Synthesize 25 nt RNA oligo, and connect the 5' phosphate end of the single-stranded RNA to the 3' hydroxyl end through ligase to form a circular structure.
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Left: LC-MS during the cyclization reaction | Right: LC-MS after the cyclization reaction | |
Fig. 1 The target molecular weight before and after cyclization differs by 17.6, which is consistent with the characteristics of linearized RNA cyclization. During the cyclization of linearized RNA, an additional phosphodiester bond is formed, and a water molecule is removed, resulting in a decrease in molecular weight of 18. | ||
| Fig. 2 Verification of RNA cyclization using linear RNA digestion enzyme. Linear and circular RNA fragments of the same size were treated with linear digestion enzyme. The linear RNA was digested, while the circular RNA showed no significant change. 1: Marker 2: Linear RNA 3: Linear RNA treated with linear digestion enzyme (digested) 4: Circular RNA 5: Circular RNA treated with linear digestion enzyme (undigested) | |
Circular Oligonucleotide Synthesis
Explore GenCefe’s circular oligonucleotide synthesis solutions, including circular ssDNA and circular RNA (circRNA), supported by flexible synthesis technologies for diverse research needs.
