In next-generation sequencing (NGS), oligos  (short for oligonucleotides) have various applications, including:

1)  Targeted Sequencing: Oligos are used to design primers for selective amplification of specific DNA or RNA sequences, enabling the study of particular genes, genomic regions, or transcripts.

2)  Library Preparation: Oligos are employed to label DNA or RNA fragments during library preparation processes, facilitating library construction and enrichment.

3)  Polymerase Chain Reaction (PCR): Oligos serve as primers in PCR to amplify target DNA segments, playing a crucial role in DNA replication and amplification.

4)  RNA Capture and Sequencing: Oligos are utilized to capture RNA, such as reverse transcribing RNA and using specific oligos for library enrichment, enabling RNA sequencing analysis.

5)  Molecular Barcoding: Oligos can function as molecular barcodes, tagging samples for differentiation in high-throughput sequencing, and aiding in sample tracking and data analysis.

6)  Single Cell Sequencing: In single-cell sequencing, oligos are used to label RNA or DNA of individual cells, facilitating identification and differentiation during subsequent sequencing.

7)  SNP Genotyping: Oligos are used for the detection and genotyping of single nucleotide polymorphisms (SNPs), applicable in genetic disease research, population genetics, among other fields.

8)  DNA/RNA Hybridization: Oligos are employed in hybridization experiments to bind with target DNA or RNA sequences, useful for detection, separation, and quantification of specific sequences.

9)  DNA/RNA Aptamers: Oligos are used for screening and constructing DNA or RNA aptamers, high-selectivity molecular probes capable of binding to specific molecules or proteins.

10)  Epigenetic Analysis: Oligos are utilized for the enrichment and detection of methylation or histone modification sites, aiding in the study of epigenetic changes.

GenCefe Biotech’s professional technical team utilizes our advanced oligo synthesis and modification  technology platform to provide adapters for NGS library construction, blockers and capture probes for hybridization capture, and primers for multiplex PCR. We are committed to supporting your NGS experiments with high-quality primers and probes .