Protein Purification—Protein purity requirements for different experiments

What are the differences in protein purity requirements for different experiments?

When conducting various experiments, the required purity of proteins can vary significantly depending on the specific goals, detection methods, and downstream applications. Below is a table summarizing the protein purity requirements for different types of experiments:

Experiment Type	Protein Purity Requirement	Reason
Protein Structure Analysis	>95%	To ensure accurate structural data and minimize interference from impurities.
Enzymatic Analysis	85%-95%	To ensure accurate measurement of enzymatic activity without interference from impurities.
Antibody Production	70%-80%	Sufficient purity for immunization, with some tolerance for impurities.
Protein Function Studies	>85%	To ensure that the observed function is related to the target protein, not impurities.
Mass Spectrometry (MS)	>90%	Impurities may interfere with data interpretation and quantification.
Cellular Function Studies (e.g., Transfection, RNAi)	70%-90%	Moderate levels of impurities are acceptable depending on the specific experiment.
Drug Development and Clinical Trials	>98%	To ensure drug safety and efficacy, minimizing the risk of side effects.
Standard Protein Preparation (e.g., ELISA Standards)	>95%	High purity is essential for accurate detection in assays.

GenCefe provide one-stop solutions from codon optimization, gene synthesis, to protein expression and purification. In addition to four conventional expression systems of bacterial, yeast, insect cell, and mammalian cell, we have also developed a patented cell free expression technology, which is suitable for the production of difficult proteins such as toxic proteins and membrane proteins.

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