Oligos  have several applications in quantitative polymerase chain reaction (qPCR), including:

1)  Primer Design: Oligos are crucial components in qPCR primer design. They are designed to anneal to specific regions flanking the target DNA sequence, enabling selective amplification during qPCR.

2)  Probe Design: Oligos are used to design fluorescent probes for qPCR assays. These probes typically consist of a fluorescent dye and a quencher molecule, and they hybridize to the target DNA sequence during qPCR, allowing for real-time monitoring of amplification.

3)  Multiplexing: Oligos enable the multiplexing of qPCR reactions, where multiple target sequences can be amplified simultaneously in a single reaction using different sets of primers and probes labeled with distinct fluorescent dyes.

4)  Detection of Gene Expression: Oligo-based qPCR assays are widely used for gene expression analysis. By designing oligo primers and probes specific to target genes, qPCR can quantitatively measure the expression levels of those genes in biological samples.

5)  Allelic Discrimination: Oligos can be designed to distinguish between different alleles of a gene in qPCR assays, enabling the detection of single nucleotide polymorphisms (SNPs) or mutations associated with genetic diseases or traits.

6)  Absolute Quantification: Oligo-based qPCR can be used for absolute quantification of target nucleic acids by comparing the amplification curves of unknown samples to standard curves generated from known concentrations of reference nucleic acids.

7)  Pathogen Detection: Oligo-based qPCR assays are employed for the sensitive and specific detection of pathogens such as viruses, bacteria, and fungi in clinical samples, environmental samples, and food samples.

Overall, oligos  play a crucial role in primer and probe design, enabling the specificity, sensitivity, and versatility of qPCR assays for various applications in molecular biology, diagnostics, and research.

GenCefe Biotech has established an advanced oligo synthesis and modification technology platform with an experienced technical team to provide customized qPCR primers and probes. We are committed to assisting your research and diagnostic applications with high accuracy and reliability of qPCR primers and probes .