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GenCefe's scientific team has developed a proprietary cell-free protein expression technology platform to solve the problem of expression of special proteins, such as membrane proteins and toxic proteins. Our team has rich practical experience in GPCR, ion channel proteins, aquaporins, and transport proteins. We provide flexible services to meet customers' specific needs.
Cell-free protein expression system is an in vitro protein expression technology based on cell extracts. In vitro synthesis uses exogenous mRNA or DNA as the template, and artificially controls the substrates, transcription and translation related factors required for protein synthesis to achieve in vitro synthesis of the target protein. Due to the simple genetic background and convenient use, cell free protein expression has become an important technology for various applications. It is especially suitable for small and medium-scale protein expression, high-throughput expression screening, and difficult membrane protein production.
Service Specifications：
| Service | Specifications |
| Membrane protein production with cell free system |
Gene synthesis with codon optimization, vector construction, purification tag design Expression level: Mg2+, K+ screening, membrane protein up to 1 mg and above Expression condition optimization: detergent, liposome, Nanodisc, stabilizer selection Sample characterization: SDS-PAGE, Westernblot, Geel-filtration Functional assays: ligand binding (SPR, QCM-D, NMR) |
| Customized membrane protein preparation |
C13, N15 labeled protein sample preparation for NMR (solid state/solution state NMR) Amino acid labeling (selective labeling/combinatorial labeling) Insertion of unnatural amino acid |
| Full-length membrane protein antigen preparation |
Efficient synthesis of full-length membrane proteins Screening of soluble conditions |
| Membrane Proteins |
GPCR: ETA, ETB, beta1-adrenergic receptor, bacteria-Rhodopsin, proteo-Rhodopsin Aquaporin: AqpZ; mAQP4; AQP1 Ion Channel: KcsA Bacteria toxin: alpha-hemolysin |
| Customized cell lysate |
S12, S30, S60, S100, S150 Various E.coli strain: A19, D10, BL21, Rosetta |
Cell Free Expression Workflow
Note: Large scale bacterial fermentation service, higher purity, label removal and endotoxin removal services are available upon request. Please contact our PM team ( [email protected] ) for details.
| REQUEST A QUOTE | ||
|---|---|---|
| PHONE | ONLINE FORM | |
| [email protected] | +1 408-828-0438 | ONLINE QUOTE SUBMISSION |
