Vaccine and Novel Therapy
Accelerating the Development of RNA Vaccines and Genetic Medicines
Vaccine and cell/gene therapy (CGT) innovations are transforming the future of medicine. Technologies such as mRNA vaccines, CAR-T therapies, and viral vector-based gene delivery rely on precise nucleic acid design, efficient expression systems, and high-quality template preparation. From synthetic gene design to mRNA production and early-stage validation, researchers need flexible, scalable partners to support rapid iteration and experimental success.
GenCefe Biotech offers integrated solutions for the research and preclinical development of RNA-based vaccines, gene therapies, and cell therapies. Our platform combines advanced DNA synthesis, high-quality plasmid preparation, in vitro transcription (IVT) of mRNA, and fast expression validation to help you bring concepts closer to clinic-ready materials.
Application Scenarios in Vaccine and CGT Research：
Application | User Needs | GenCefe Solutions |
mRNA Vaccine Development (Infectious Disease, Oncology) | Design, produce, and test mRNA encoding viral antigens or tumor-associated proteins. | - Gene synthesis with codon optimization, UTR design |
Gene Therapy Research (AAV, Lentiviral Systems) | Construct and produce plasmids encoding gene payloads or vectors for delivery. | - Custom gene synthesis for therapeutic payloads or regulatory elements |
Cell Therapy Tools (e.g., CAR-T) | Synthesize and validate constructs for chimeric receptors, editing tools, or signaling modules. | - Sequence design and synthesis for CARs, cytokines, Cas variants |
Functional Validation & Screening | Rapidly test new constructs for protein expression and immune activity. | - Cell-free expression of antigens, cytokines, Cas proteins |
GenCefe Core Service Modules | Supporting Vaccine & CGT Innovation
· Gene Synthesis: High-accuracy synthesis of antigen genes, therapeutic transgenes, CAR sequences, regulatory elements, and viral payloads. Codon optimization and GC-balancing included.
· Plasmid Construction & Preparation: Research to transfection grade plasmid construction and production with optional multi-cassette support for gene therapy and vaccine plasmids. Delivered with sequence validation and QC report upon request.
· IVT mRNA Synthesis: mRNA synthesis with options for Cap 0/Cap 1, modified bases (Ψ, m5C), poly(A) tail, and UTR optimization. DNA templates can be synthesized or provided by clients.
· ssDNA & ssRNA Synthesis: Custom single-stranded DNA/RNA for use as HDR templates, donor strands, antisense oligos, or control molecules. Available with chemical modifications.
· Oligo Synthesis: High-quality DNA/RNA oligos including primers, probes, sgRNAs, and therapeutic oligos with customizable modifications (5’, 3’, LNA, biotin, etc.).
· Cell-Free Protein Expression: Fast expression of vaccine antigens, membrane or toxic proteins, and difficult-to-express constructs without cell culture. Ideal for early-stage screening and validation.
