Gene&Genome Editing
High-Performance Tools for CRISPR and Beyond
As CRISPR/Cas and related genome editing technologies continue to expand across research, therapeutics, and synthetic biology, demand grows for highly reliable, customizable editing components. Whether targeting single genes or conducting genome-scale functional screens, researchers rely on precise editing tools—particularly high-quality guide RNAs, vectors, donor templates, and pooled libraries.
GenCefe Biotech offers a full portfolio of gene editing tools designed for flexibility, accuracy, and scalability. Our services cover sgRNA synthesis (short and long format), custom vector construction, high-diversity sgRNA libraries, and ssDNA/ssRNA donor synthesis, along with supporting services such as oligo and gene synthesis.
Application Scenarios in Gene Editing:
Application | User Needs | GenCefe Solutions |
CRISPR Knockout & Gene Disruption | Robust delivery of high-quality sgRNAs for gene knockout or transcriptional silencing. | - Chemically synthesized short sgRNAs or sgRNA+scaffold fragments |
CRISPR Knock-In & HDR Repair | Precision insertion of DNA via HDR requires matched sgRNA and donor template. | - Dual sgRNA design and synthesis |
sgRNA Library Screening (KO, CRISPRa/i) | High-throughput gene function studies with pooled sgRNA libraries. | - Custom sgRNA library design and oligo pool synthesis |
Editing Efficiency Verification | Rapid testing of sgRNA cleavage efficiency before full experiment deployment. | - sgRNA fragments for IVT and in vitro Cas9 cleavage |
Synthetic Biology & Regulatory Element Editing | Multi-site targeting or logic-based gene control systems. | - Multi-guide constructs or modular vector assembly |
GenCefe Core Tool Services | Supporting Your Editing Platform
· sgRNA Synthesis: Short sgRNAs (20–22 nt) and long sgRNA+scaffold fragments (80+ nt), suitable for direct use in IVT, RNP, or vector workflows.
· sgRNA Library Construction: Custom-designed pooled sgRNA libraries for genome-wide or targeted gene sets, available as oligo pools, cloned plasmids, or lentiviral vectors.
· Vector Construction: Ready-to-use CRISPR vectors including single or dual sgRNA, Cas variants, HDR templates, and regulatory elements. End-to-end design and cloning support.
· ssDNA & ssRNA Synthesis: Single-stranded DNA/RNA synthesis for HDR templates, donor strands, antisense RNA, or control sequences. Modifications and purification available.
· Oligo Synthesis: High-quality DNA oligos for PCR, cloning, IVT templates, and T7 promoters—available with 5'/3' modifications or labels.
· Gene Synthesis: Optimized gene fragments or full-length sequences for Cas proteins, base editors, donor inserts, and regulatory elements.
· Cell-Free Expression & Cleavage Validation: In vitro CRISPR/Cas testing systems for sgRNA screening or validating editing outcomes without live cell systems.
