gRNA Synthesis
GenCefe Biotech provides high-quality, customizable gRNA synthesis services, including single guide RNA (sgRNA) for CRISPR-Cas9 applications and primer-editing guide RNA (pegRNA) for precise genome modification. We offer flexible synthesis options to meet diverse research needs—supporting sequences up to 300 nucleotides, multiple delivery formats, and custom designs for commonly used species.
Whether you need a standard gRNA or an extended sequence with a complex structure, our portfolio of advanced synthesis platforms ensures reliable performance and superior purity. All products are manufactured under strict quality systems to support research and regulatory environments.
Why Choose GenCefe?
Wide Range of Lengths and Structures: Custom synthesis of sgRNA and pegRNA up to 300 nt, with or without scaffold extensions.
Flexible Delivery Formats: gRNAs are available as single-stranded RNA, RNA duplexes, or vector-based constructs, delivered in lyophilized or solution form.
Design Support for Common Species: Sequence design assistance is available for commonly studied organisms to help you streamline your project.
ISO-Certified Quality Systems: All gRNA products are produced under ISO 9001 and ISO 13485 certifications, ensuring consistency and traceability.
Service Specifications:
Length | Purification Method | Turnaround Time | Deliverables | |
gRNA (sgRNA & pegRNA) | ~20 nt | Desalting | 4-6 days | Lyophilized gRNA COA Report |
HPLC | 5-7 days | |||
90-110 nt | Desalting | 8-12 days | ||
HPLC | ||||
>110 nt | HPLC | |||
Plasmid | ~20 nt (inserted gRNA) | / | 8-12 business days | 4 μg plasmid containing the target gRNA |
