DNA Oligo QC

GenCefe Biotech has established a strict quality control system for oligonucleotide products and has passed ISO9001 certification. In addition to standard QC items, we also provide comprehensive customized QC items for different applications, such as scientific research and in vitro diagnostics.

Oligo Detection Methods:

Detection Items (methods)	Equipment	Brief Introduction
Appearance	30x magnifying glass inspection lamp	Detect the presence of undried product, impurities, etc.
Quantitative (UV)	UV spectrophotometer	Detect the quantitative of Oligos
Molecular weight (MS)	Electron Spray Mass Spectrometry	Detect the molecular weight of Oligos
Purity (HPLC)	High performance liquid chromatography	Detect the purity of Oligos
Purity (CE)	Capillary electrophoresis
Purity (PAGE)	Vertical gel electrophoresis
CT value(qPCR)	Fluorescent quantitative PCR	Detect the positive target detection capabilities of the qPCR primer & probes
Fluorescence value(qPCR)		Detect the fluorescence value of qPCR primer & probes when amplifying positive targets
Human genomic DNA contamination (qPCR)		Detection of human genomic DNA contamination in qPCR primer & probes.
NTC amplification (qPCR)		Detect abnormal amplification signals of qPCR primer & probes in a template-free system.
Cross Contamination Rate (NGS)	High throughput sequencer	Detect the probability of cross-contamination between different oligos

Oligo QC Items:

Detection Item	Primer Type	Primer	qPCR Primer	qPCR Probe	NGS Primer	Note
Property detection	Appearance	Dry powder: uniform powder or transparent solid, without obvious impurities				Standard Detection
		Liquid: colorless/specific color liquid, clear and transparent, without obvious impurities
	Quantitative (UV)	Error rate(％)≤±10％
	Molecular weight (MS)	Error rate(％)s＋0.05％
	Purity (HPLC)	RPC≥85%;MOP≥90%;PAGE≥95%;HPLC/HPLC IVD≥95%				Customize Detection
	Purity (CE)	Vary according to the purification method and the oligo length
	Purity (PAGE)	Clear main product band, correct molecular weight, no obvious impurities
Function Detection	Ct Value (qPCR)	-	A normal amplification curve can be detected within 45 cycles, and the Ct value is within a reasonable range			Customize Detection
	Fluorescence value(qPCR)	-	According to the agreement
Contamination Detection	Human genomic DNA contamination (qPCR)	-	No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40
	NTC amplification (qPCR)	-	No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40
	Cross Contamination (NGS)	-	-	-	Cross Contamination Rate(%)≤0.1%

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EMAIL	PHONE	ONLINE FORM
[email protected]	+1 408-828-0438	ONLINE QUOTE SUBMISSION

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