Mutagenesis

Custom DNA mutagenesis for precise sequence modifications.

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Mutagenesis

GenCefe provides site-directed mutagenesis services for precise DNA sequence modifications, including insertions, deletions, and substitutions. Our scientific team can introduce specified mutations into target DNA sequences and deliver sequence-verified mutant constructs. This service is particularly useful when researchers need to change specific amino acids or introduce targeted mutations in an existing construct, or generate multiple mutant variants for functional comparison. Mutagenesis can be provided as a standalone service or combined with gene synthesis, depending on project requirements. 

Key Features

  • Multiple Mutation Types: Support targeted DNA insertions, deletions, and substitutions according to project requirements.

  • Flexible Mutation Design: Introduce specified mutations at target sites, including changes designed to modify specific amino acids or generate mutant variants for functional comparison.

  • Gene Synthesis Integration: Combine site-directed mutagenesis with gene synthesis when broader sequence modifications or new constructs are required.

  • Integrated Construct Support: Connect mutagenesis with gene synthesis and plasmid preparation to support the development of sequence-verified DNA constructs within a streamlined workflow.

Site-directed Mutagenesis Service Specifications

Service Type

Gene Length

Mutated Site

Turnaround Time (Business Day)

Price

Site-Directed Mutagenesis bundled with gene synthesis

< 3000 bp

One

5 Business Days

$89/mutation

Two

7 Business Days

$139/mutation

Three

7 Business Days

$189/mutation

>3000 bp

One

7 Business Days

Get a Quote

Two

9 Business Days

Get a Quote

Three

9 Business Days

Get a Quote

Site-Directed Mutagenesis Only

< 3000 bp

One

5-8 Business Days

$89/mutation

Two

10-14 Business Days

$139/mutation

Three

15-20 Business Days

$189/mutation

>3000 bp

One

5-8 Business Days

Get a Quote

Two

10-14 Business Days

Get a Quote

Three

15-20 Business Days

Get a Quote


Delivery Specifications

  • 4 μg of lyophilized plasmid

  • Electronic sequence chromatograms covering the target gene

  • Electronic plasmid construct map

  • Quality assurance certificate

Mutant constructs are sequence-verified to confirm the intended sequence.

Technical Resources
Gene Synthesis Services

Gene Synthesis Services

Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.

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Plasmid Preparation
GenCefe provides high-quality plasmid preparation from micrograms to grams, with strict QC for batch consistency.
Discuss Your Project with GenCefe’s Scientific Team
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Discuss your mutagenesis project with GenCefe’s scientific team and get support tailored to your sequence requirements.
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+1 408-828-0438