GenCefe provides site-directed mutagenesis services for precise DNA sequence modifications, including insertions, deletions, and substitutions. Our scientific team can introduce specified mutations into target DNA sequences and deliver sequence-verified mutant constructs. This service is particularly useful when researchers need to change specific amino acids or introduce targeted mutations in an existing construct, or generate multiple mutant variants for functional comparison. Mutagenesis can be provided as a standalone service or combined with gene synthesis, depending on project requirements.
Key Features
Multiple Mutation Types: Support targeted DNA insertions, deletions, and substitutions according to project requirements.
Flexible Mutation Design: Introduce specified mutations at target sites, including changes designed to modify specific amino acids or generate mutant variants for functional comparison.
Gene Synthesis Integration: Combine site-directed mutagenesis with gene synthesis when broader sequence modifications or new constructs are required.
Integrated Construct Support: Connect mutagenesis with gene synthesis and plasmid preparation to support the development of sequence-verified DNA constructs within a streamlined workflow.
Site-directed Mutagenesis Service Specifications
Service Type | Gene Length | Mutated Site | Turnaround Time (Business Day) | Price |
Site-Directed Mutagenesis bundled with gene synthesis | < 3000 bp | One | 5 Business Days | |
Two | 7 Business Days | |||
Three | 7 Business Days | |||
>3000 bp | One | 7 Business Days | ||
Two | 9 Business Days | |||
Three | 9 Business Days | |||
Site-Directed Mutagenesis Only | < 3000 bp | One | 5-8 Business Days | |
Two | 10-14 Business Days | |||
Three | 15-20 Business Days | |||
>3000 bp | One | 5-8 Business Days | ||
Two | 10-14 Business Days | |||
Three | 15-20 Business Days |
Delivery Specifications
4 μg of lyophilized plasmid
Electronic sequence chromatograms covering the target gene
Electronic plasmid construct map
Quality assurance certificate
Mutant constructs are sequence-verified to confirm the intended sequence.

Gene Synthesis Services
Explore GenCefe’s gene synthesis capabilities, including custom synthesis options, sequence optimization, quality control, and service workflows.








