IVT mRNA Synthesis
High-quality mRNA serves as a crucial tool in various scientific, medical, and biotechnological applications, especially in the mRNA vaccine and therapy development. The reliability and purity of the mRNA are essential for achieving accurate and reproducible results. In vitro transcribed (IVT) mRNA synthesis lies in its ability to provide researchers and biotechnologists with a customizable, rapid, and controlled method for generating high-quality mRNA molecules for diverse applications.
At GenCefe Biotech, we specialize in providing cutting-edge IVT mRNA synthesis services. Our state-of-the-art facilities and experienced team ensure the production of high-quality synthetic mRNA tailored to meet your specific research or discovery needs.
Service Features
One-stop solution: from codon optimization , gene synthesis , plasmid preparation , to in vitro transcription of mRNA and purification.
Flexible synthesis specifications: a variety of modifications, capping, quantity, purity are available.
Strict quality control: standard QC items and a variety of customized QC items can be provided according to customer requirements.
Service Workflow
5’ Cap | Poly A Tail | Modifications |
Cap0 (m7GpppN) | 110±5 nt A Tail, | Pseudouridine |
Service Specifications
Step | Description | Turnaround Time | Deliverables | Order |
Gene Synthesis | Codon optimization | Starting from 2 weeks | Sanger sequencing result for the synthesized gene | |
Plasmid Preparation | Plasmid preparation | |||
IVT mRNA | 20 μg-2mg | 1-2 weeks | ||
5 mg | 2-3 weeks | |||
>5 mg | ||||
Standard QC items | appearance, mRNA length, sequence accuracy, RNA concentration, A260/280, purity | / | CoA | |
Customized QC items (optional, extra charge) | PolyA tailing efficiency, capping efficiency, template DNA residue, protein residue, RNase residue, dsRNA detection, endotoxin content, bioburden |
* LNP-encapsulated mRNA cannot be lyophilized and can only be shipped in liquid form at low temperatures, which will incur additional dry ice and shipping costs.
Off-the-shelf mRNA Products
Product Name | Size | Turnaround Time | Order |
eGFP mRNA | 100 μg/tube | 3-5 days | |
mCherry mRNA | 100 μg/tube | 3-5 days | |
Luciferase mRNA | 100 μg/tube | 3-5 days | |
mCherry-eGFP mRNA | 100 μg/tube | 3-5 days | |
Cas9 mRNA | 100 μg/tube | 3-5 days |
