Not all proteins require signal peptides for expression. Signal peptides are short amino acid sequences that direct the transport of the protein to specific cellular compartments, such as the endoplasmic reticulum (ER) for secretion or membrane insertion. Whether a signal peptide is needed depends on the desired location and function of the expressed protein.

The molecular weight of a protein can significantly impact its expression in various expression systems. Here are several ways in which protein molecular weight can influence expression:

Selecting the appropriate recombinant protein expression system is crucial for the successful production of the target protein. Different expression systems have their own advantages and disadvantages, making them suitable for various applications. Here are the main expression systems and criteria for their selection:

Recombinant protein expression is a widely used technique in biology and biotechnology that allows the production of large quantities of target proteins in host cells. Here are the main reasons and advantages of using recombinant protein expression: