CRISPR Cas9

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CRISPR Cas9

From gene knockout to precise editing, GenCefe provides a full suite of customizable tools for CRISPR-based research — including high-quality gRNA synthesis, single-stranded DNA (ssDNA) synthesis, and sgRNA libraries for high-throughput screening.

Our services are built on deep technical expertise and advanced synthesis platforms, offering the flexibility, precision, and scalability your genome editing projects demand.

Accelerate your genome engineering with GenCefe’s comprehensive CRISPR solutions.


Guide RNA Tools and Recommended Applications

Type

Format

Typical Applications

sgRNA

Single guide RNA (short)

Standard gene knockout, CRISPRi/CRISPRa, transient transfection

sgRNA + Scaffold

Extended guide RNA with scaffold

In vivo editing, long-target sequences, synthetic circuits

sgRNA Plasmid

sgRNA cloned into expression vector

Stable expression, lentiviral delivery, long-term or in vivo editing

pegRNA

Prime editing guide RNA with extension

Precise base editing, targeted insertions/deletions without DSBs

sgRNA Library

Pooled or arrayed sgRNA collections

Genome-wide screening, functional genomics, pathway mapping

 

Service Features

●  High-purity synthesis for long gRNAs and ssDNAs

●  Flexible designs: single, pooled, and vector formats

●  ISO 9001 & ISO 13485 certified manufacturing standards

●  Comprehensive CRISPR toolkit with expert technical support


gRNA Synthesis
GenCefe Biotech provides high-quality, customizable gRNA synthesis services, including single guide RNA (sgRNA) for CRISPR-Cas9 applications and primer-editing guide RNA (pegRNA) for precise genome modification.
ssDNA Synthesis Services
GenCefe Biotech offers high-purity ssDNA synthesis up to several kb, with no double-strand residues—ideal for cell-based and in vivo research applications.
sgRNA library
CRISPR sgRNA libraries serve as powerful tools for high-throughput genomic screening. By silencing or activating gene expression on a large scale, the link between genes and phenotypes can be established, and the genes responsible for specific phenotypes can be identified.
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