Mammalian expression systems use various tags to facilitate purification, detection, and visualization of recombinant proteins. Here are some commonly used tags:
Mammalian cell expression systems are widely used for producing recombinant proteins, especially those requiring complex post-translational modifications. Here are some commonly used mammalian cell types and their characteristics:
In insect expression systems, specific tags are employed to improve the solubility and facilitate the purification of recombinant proteins. These tags help in overcoming challenges associated with protein aggregation and provide convenient methods for isolating the target protein from complex mixtures. Here are some commonly used tags:
1. Sf9 Cells: Derived from Spodoptera frugiperda (fall armyworm) ovary cells, they are commonly used in the baculovirus expression system for high levels of recombinant protein production.
Insect expression systems, particularly the baculovirus system, are widely used for recombinant protein production due to their ability to express complex proteins with eukaryotic modifications. Here are some key characteristics: