Peptide Synthesis: 

Certain proteins are challenging to express due to a combination of intrinsic properties of the protein and limitations of the expression system. The main reasons include: 

In transient expression, various parameters can be optimized to enhance protein yield, transfection efficiency, and overall expression performance. Key parameters include: 

Transient Expression: 

Cleavage sites are used to remove tags from recombinant proteins after purification to obtain the native protein. Here are some commonly used cleavage sites: