While traditional CRISPR-Cas9 systems have transformed genome editing, they often rely on double-strand breaks and donor templates, which can introduce unwanted errors. Prime Editing emerged as a next-generation approach, offering higher precision and versatility. Central to this method is the prime editing guide RNA, or pegRNA.

What is PegRNA?

PegRNA is a specialized guide RNA that extends beyond the standard sgRNA structure. In addition to directing the Cas9 nickase to the target site, pegRNA carries a primer binding site and a reverse transcriptase template sequence. This unique design allows precise insertion, deletion, or correction of genetic information without double-strand breaks.

How PegRNA Works in Prime Editing

In Prime Editing, Cas9 nickase makes a single-strand cut, while the reverse transcriptase uses the pegRNA template to write the desired genetic change directly into the DNA. This method reduces reliance on homology-directed repair and minimizes unintended mutations, making it a powerful alternative for precise genome modifications.

Advantages of PegRNA in Gene Editing

PegRNA enables:

●  High precision in correcting point mutations.

●  Reduced off-target risks compared to standard CRISPR methods.

●  Expanded editing scope, including small insertions, deletions, and replacements.

●  Potential therapeutic applications in genetic diseases where precise correction is essential.

Research Applications of PegRNA

PegRNA has already shown promise in multiple areas:

●  Genetic disease modeling, including sickle-cell disease and cystic fibrosis.

●  Induced pluripotent stem cells (iPSC) research for regenerative medicine.

●  Drug discovery and validation, where accurate genetic modifications are needed.

PegRNA represents a major advance in the CRISPR toolkit, transforming Prime Editing into a precise and versatile platform.

GenCefe Solutions for PegRNA Synthesis

At GenCefe Biotech, we provide custom pegRNA synthesis with tailored lengths, sequence designs, and optional modifications. Our rigorous QC ensures reliability, enabling researchers to confidently apply Prime Editing in their projects.