Synthetic DNA Libraries

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Synthetic DNA Libraries

The synthetic DNA library is a combination of a large number of DNA mutant sequences constructed to screen mutant proteins with specific functions. It has been widely used in research fields such as protein directed evolution and antibody screening. GenCefe Biotech's experienced R&D and production team relies on the advanced AI bioinformatics platform and gene synthesis technology platform to provide customized synthetic DNA library services. We can mutate the amino acid or nucleotide sequences specified by customers, or design and synthesize mutation libraries according to customer requirements, such as site-directed mutagenesis libraries, random mutant libraries, degenerate mutation libraries, controlled libraries, sgRNA libraries and other types of library construction services.


Our Services

Library TypeRecommended ApplicationsDetails
Site-directed mutagenesis libraries 1. Research on protein structure and function
2. Study of the enzyme active sites
3. Study on the antibodies binding domains
4. Protein property modifications, such as changing thermal stability, substrate binding specificity, etc.
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Random mutant library 1. Research on protein structural functional domains;
2. Antibody humanization;
3. Protein property modifications, such as the determination of the catalytic domain of the enzyme, the improvement of catalytic properties, etc.
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Degenerate mutation libraries1. Research on protein structural functional domains;
2. Directed evolution of proteins, enzymes, antibodies;
3. Determination and modification of key amino acids in enzyme active sites;
4. Antibody humanization, fine-tuning of antibody affinity and binding properties.
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Controlled libraries 1. Directed evolution of proteins, enzymes, antibodies;
2. New drug target screening;
3. In-depth study of the effect of a few amino acids around the catalytic site of the enzyme on the catalytic properties of the enzyme, etc.
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sgRNA Libraries1. Large-scale gene knockout, activation and suppression
2. Identification and verification of new drug targets
3. Identify cancer treatment targets
4. Applications in agricultural field, such as development of disease-resistant crops
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Site-Directed Mutagenesis Libraries
Site-directed mutagenesis libraries refer to a group of gene sequences with mutated amino acids formed by replacing one or more amino acids encoding proteins or peptides with specified amino acids.
Random Mutant Library
The random mutation library refers to the random introduction of nucleotide mutations in the gene sequence by a specific method, thereby generating a mutant gene mixture containing random nucleotide mutations.
Degenerate Mutation Libraries
A degenerate mutation library, also known as a site-directed saturation library, refers to the introduction of NNK or NNN at single or multiple positions in a gene sequence.
Controlled Library
Controlled libraries, also known as site-directed unsaturation libraries or Trimer libraries, are a class of libraries similar to degenerate libraries.
SgRNA Libraries
CRISPR sgRNA libraries serve as powerful tools for high-throughput genomic screening. By silencing or activating gene expression on a large scale, the link between genes and phenotypes can be established, and the genes responsible for specific phenotypes can be identified.
Service Features
Mutagenesis library, sgRNA library, controlled libraries, degenerate library, etc.
Flexible codon control and mutation site selection to fit your goals.
Advanced technology platform ensures high-quality, low-error DNA libraries
Professional pre-sales consultation and after-sales service, timely updates
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Phone
+1 408-828-0438