miRNA (microRNA) is a class of small non-coding RNA molecules that play a critical role in regulating gene expression. Due to its functions and characteristics, miRNA has been widely applied in various fields. Below are its main applications: 

siRNA (small interfering RNA) is a powerful tool for gene silencing, and it has a wide range of applications in both research and therapeutic contexts. Here are the key applications: 

siRNA (small interfering RNA) and sgRNA (single guide RNA) are both short RNA molecules involved in RNA-mediated gene silencing, but they function in different RNA interference pathways and have distinct roles. Here are the key differences:

Assessing the silencing effect of siRNA is crucial to confirm its effectiveness in downregulating target gene expression. Several methods can be used to evaluate the silencing efficiency of siRNA. Below are the most common approaches: 

Designing effective siRNA involves several important factors to ensure high silencing efficiency, specificity, and minimal off-target effects. Below are the key considerations when designing siRNA: