1.  His-Tag (Polyhistidine Tag): 

Protein purification is a critical step in producing recombinant proteins for research, industrial, or therapeutic applications. The goal is to isolate the target protein from a complex mixture of other proteins and impurities. Here are some common protein purification methods:

Choosing the right signal peptide is critical for efficient and accurate protein targeting. Here are some factors to consider: 

Not all proteins require signal peptides for expression. Signal peptides are short amino acid sequences that direct the transport of the protein to specific cellular compartments, such as the endoplasmic reticulum (ER) for secretion or membrane insertion. Whether a signal peptide is needed depends on the desired location and function of the expressed protein.

The molecular weight of a protein can significantly impact its expression in various expression systems. Here are several ways in which protein molecular weight can influence expression: