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It targets the SMN2 gene to enhance functional SMN protein production. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Mipomersen (Kynamro): Used to treat familial hypercholesterolemia by reducing apolipoprotein B (ApoB) production. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Eteplirsen (Exondys 51): Targets exon skipping in the dystrophin gene for Duchenne muscular dystrophy (DMD). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. Expanding Disease Applications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Neurological Diseases: ASOs have shown promise in treating Huntington’s disease (e.g., Tominersen) and amyotrophic lateral sclerosis (ALS). These drugs are designed to reduce harmful protein accumulation or correct splicing defects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Oncology: ASOs are being investigated to silence oncogenes or re-sensitize tumors to chemotherapy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Metabolic Disorders: Targeting liver-specific pathways, ASOs like inclisiran reduce low-density lipoprotein (LDL) cholesterol. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. Innovations in Chemistry \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Chemical modifications have improved ASO stability, delivery, and efficacy: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Phosphorothioate Backbones: Enhance stability and resistance to nucleases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      2'-O-Methyl (2'-OMe) and 2'-Methoxyethyl (2'-MOE): Increase binding affinity and reduce toxicity. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Gapmers: ASOs designed to activate RNase H-mediated cleavage, improving potency. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Delivery Advancements \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Targeted Delivery: Liver-specific delivery through conjugates like N-acetylgalactosamine (GalNAc) has reduced off-target effects and improved safety profiles. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Intrathecal Administration: Direct delivery to the central nervous system (CNS) for neurological diseases, bypassing the blood-brain barrier. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. Ongoing Clinical Trials \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Dozens of ASO drugs are in clinical development, targeting diseases such as Alzheimer’s, cancer, and rare genetic conditions. Emerging therapies include RNA-targeting ASOs for exon skipping, splicing correction, and gene silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">ASO drug development has achieved remarkable progress, with approved therapies addressing rare genetic diseases and expanding into more prevalent conditions like cancer and neurological disorders. Advances in chemistry, delivery systems, and disease-specific targeting are driving the growth of ASOs as a transformative therapeutic platform.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-02-27 00:00:00","RNA Synthesis & Modifications—Progress in antisense oligonucleotide (ASO) drug development"," ASO drug development has achieved remarkable progress, with approved therapies addressing rare genetic diseases and expanding into more prevalent conditions like cancer and neurological disorders.","RNA Oligo, ASO, antisense oligo, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:50:17","67,66,65,64,63","13,60",[301,312,322,332,342],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":126,"categoryName":289,"title":302,"imgUrl":303,"content":304,"excerpt":78,"publishDate":305,"seoTitle":306,"seoDesc":307,"seoKeywords":308,"createDate":309,"sort":185,"relatedPostIds":310,"firstServiceProductId":49,"secondServiceProductIds":311,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"What are the key considerations in siRNA design?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F10_20260630145140A109.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F10_20260630145147A110.png\" title=\"key considerations\" alt=\"key considerations\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Designing effective \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> involves several important factors to ensure high silencing efficiency, specificity, and minimal off-target effects. Below are the key considerations when designing siRNA: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1. Target Sequence Selection \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Select an Efficient Target Region: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Choose a target sequence that is highly conserved among species and is ideally located in the 3' untranslated region (UTR) or coding region to maximize gene silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Avoid Off-Target Sequences: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Ensure that the siRNA sequence does not significantly match other genes in the genome to reduce off-target effects. Tools like BLAST can be used to assess off-target potential. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Preferably Avoid Sequence with Secondary Structures: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Select target sequences that do not form strong secondary structures (such as hairpins), as these can hinder the binding of the siRNA to the mRNA. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. siRNA Duplex Design \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Guide and Passenger Strand Balance: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">The guide strand is the one that binds to the target mRNA, while the passenger strand is degraded. Ensure that the guide strand is selected from the most potent position, usually the middle of the target sequence. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    The guide strand should have a 5' phosphate, and the passenger strand should have a 3' overhang for efficient loading into the RNA-induced silencing complex (RISC). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Strand Stability: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Ensure a proper balance in the melting temperature (Tm) between the two strands. The 5' end of the guide strand should ideally be more stable than the passenger strand to promote unwinding of the duplex and incorporation of the guide strand into RISC. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. siRNA Length \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Typically 19-23 Nucleotides: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">siRNA is typically designed to be 19-23 base pairs long. This length is optimal for efficient cleavage by the RNA-induced silencing complex (RISC) and minimizes the risk of immune activation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Chemical Modifications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Phosphorothioate Backbone Modification:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Introduce phosphorothioate modifications at the 3' and\u002For 5' ends of the siRNA to increase stability against nucleases in serum and cellular environments. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    2'-O-Methyl and 2'-Fluoro Modifications: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Use these modifications to protect the siRNA from degradation and reduce activation of innate immune responses by Toll-like receptors (TLRs). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Cholesterol Conjugation (for in vivo): \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Conjugate cholesterol to the siRNA (typically at the 3' end) to enhance cellular uptake, particularly for liver-targeted siRNAs. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. GC Content \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    GC Content of 30-50%: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Ideally, the GC content of the siRNA should be balanced to ensure optimal stability and efficient hybridization with the target mRNA. Avoid sequences that are too rich in GC, as they may form secondary structures. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6. Avoid Sequence Homology with Other Genes \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Minimize Homology with Off-Target Genes: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Ensure that the siRNA sequence does not have significant homology with unintended genes to minimize off-target silencing effects. This can be assessed through bioinformatics tools to check for off-target sequences. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7. Avoid Immunostimulatory Sequences \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Avoid PAMP Motifs: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Avoid sequences that might trigger immune responses (e.g., patterns recognized by Toll-like receptors or other immune sensors). This can be especially critical for in vivo applications where systemic immune activation could limit efficacy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8. 5’ and 3’ End Modifications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    3' Overhang: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">For efficient incorporation into the RISC complex, siRNAs typically have a 3' overhang of 2–3 nucleotides. This facilitates the unwinding of the duplex and guide strand loading. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    5' Phosphate Group: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">The guide strand should have a 5' phosphate, which is essential for RISC binding and mRNA cleavage. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9. Secondary Structure Considerations \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Minimize Hairpin or G-Quadruplex Formation: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Avoid sequences that form strong secondary structures, such as hairpins, as these can inhibit efficient siRNA-mRNA hybridization and reduce silencing efficacy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10. Target Gene Validation \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Validate Target mRNA Structure: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Before siRNA design, ensure that the target mRNA is accessible and that there are no structural elements (like G-quadruplexes) that would prevent siRNA binding. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Efficient \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> design involves a careful balance between target specificity, stability, and minimizing immune responses or off-target effects. Key considerations include selecting optimal target sequences, modifying the siRNA backbone for stability, ensuring proper strand orientation and length, and using chemical modifications to improve pharmacokinetics, particularly for in vivo applications. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F21.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">miRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F10.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">custom RNA synthesis\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> services. We can design and synthesize RNA Oligos of different lengths, different forms, and with various modifications according to customer’s needs, and also synthesize RNA sequences designed by customers. The RNA products we deliver are all purified by HPLC. ISO 9001-certified facilities and comprehensive quality control reports ensure the delivery of high-quality products.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-02 00:00:00","RNA Synthesis & Modifications—Key considerations in siRNA design","Key considerations of siRNA design include selecting optimal target sequences, modifying the siRNA backbone for stability, ensuring proper strand orientation and length, and using chemical modifications to improve pharmacokinetics, particularly for in vivo applications.","RNA Oligo, siRNA, Antisense Oligo, Small nucleic acid drug, custom RNA synthesis, siRNA design","2025-04-30 16:33:39","77,69,62,59,57","13,22",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":130,"categoryName":289,"title":313,"imgUrl":314,"content":315,"excerpt":78,"publishDate":316,"seoTitle":317,"seoDesc":318,"seoKeywords":319,"createDate":320,"sort":45,"relatedPostIds":321,"firstServiceProductId":49,"secondServiceProductIds":311,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"How to assess the silencing effect of siRNA?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630145425A111.png","\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630145430A112.png\" title=\"silencing\" alt=\"silencing\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Assessing the silencing effect of \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002Fadmin\u002FGenCefe%20Biotech%20provides%20high-quality%20siRNA,%20miRNA,%20sgRNA,%20and%20custom%20RNA%20synthesis%20services.%20We%20can%20design%20and%20synthesize%20RNA%20Oligos%20of%20different%20lengths,%20different%20forms,%20and%20with%20various%20modification\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; font-family: Montserrat; font-size: 16px;\">siRNA\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\"> is crucial to confirm its effectiveness in downregulating target gene expression. Several methods can be used to evaluate the silencing efficiency of siRNA. Below are the most common approaches: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cbr\u002F>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">1. mRNA Expression Analysis (Quantitative PCR) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: Measure the mRNA levels of the target gene before and after siRNA treatment using quantitative PCR (qPCR). A decrease in mRNA levels indicates effective silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Ensure that the primers used are specific for the target gene and not other homologous genes to avoid off-target effects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">2. Western Blotting \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: Western blotting detects the protein levels of the target gene. If the siRNA is effective, a significant reduction in protein expression should be observed compared to the control. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Antibodies used for detection must be highly specific to the target protein. This method can be more informative than qPCR since it measures the actual protein product. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">3. Reporter Assay (Luciferase or GFP Reporter) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: If the target gene has been fused to a reporter gene (such as luciferase or GFP), the expression of the reporter can be measured. A decrease in reporter signal indicates effective siRNA silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Reporter assays are useful for measuring the impact of siRNA on specific targets, and they are relatively easy to quantify. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">4. Flow Cytometry (for Fluorescent Proteins or Surface Markers) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: If the target gene influences cell surface markers or a fluorescent protein (e.g., GFP or mCherry), flow cytometry can be used to quantify changes in expression at the single-cell level. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Flow cytometry is powerful for analyzing heterogeneous cell populations and detecting slight variations in silencing efficacy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5. RNA Sequencing (RNA-Seq) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: RNA-Seq is a high-throughput sequencing technique that can be used to evaluate changes in global gene expression profiles upon siRNA treatment. It can identify not only the target gene but also potential off-target effects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: This method provides a comprehensive view but can be more expensive and requires more extensive data analysis. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">6. Quantification of Targeted mRNA by Northern Blotting \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: Northern blotting can be used to detect and quantify specific mRNA species, including any potential cleavage products of the target mRNA. A reduction in the target mRNA band indicates effective silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Although more labor-intensive than qPCR, Northern blotting can provide additional information about the size and structure of the mRNA. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">7. Cell Phenotype Analysis \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: Analyze changes in cell phenotype (e.g., morphology, proliferation, apoptosis) after siRNA treatment, particularly if the target gene plays a role in a specific cellular process. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: This can be used as a functional readout, but it is often more difficult to directly attribute changes to specific target genes without further validation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">8. ELISA (Enzyme-Linked Immunosorbent Assay) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: If the target gene produces a secreted protein (e.g., cytokines, growth factors), ELISA can quantify the protein in the culture medium to assess the effect of siRNA on secretion. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: ELISA is useful for monitoring secreted proteins, but it requires high specificity of the antibody and the availability of commercial kits for the target protein. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">9. In Vivo Validation (Animal Models) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: In animal models, siRNA is delivered systemically or locally, and silencing effects are assessed by measuring mRNA and protein expression, or phenotypic changes (e.g., tumor growth reduction, liver disease markers). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: In vivo experiments are more complex and require ethical considerations, but they are crucial for confirming siRNA efficacy in a living organism. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">10. Cytotoxicity Assay \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Method: To ensure that the observed gene silencing is not due to cytotoxic effects, viability assays (e.g., MTT, Trypan Blue exclusion) can be used to confirm that the siRNA treatment does not cause significant cell death. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">     ●      Considerations: Always include proper controls to differentiate between silencing effects and toxic effects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cbr\u002F>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Using a combination of these methods provides a comprehensive understanding of \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002Fadmin\u002FGenCefe%20Biotech%20provides%20high-quality%20siRNA,%20miRNA,%20sgRNA,%20and%20custom%20RNA%20synthesis%20services.%20We%20can%20design%20and%20synthesize%20RNA%20Oligos%20of%20different%20lengths,%20different%20forms,%20and%20with%20various%20modification\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; text-decoration: underline; font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; font-size: 16px;\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\"> silencing efficacy and ensures that both on-target and off-target effects are carefully evaluated. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cbr\u002F>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; line-height: 1.5; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255);\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; text-decoration: underline; font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; font-size: 16px;\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F21.html\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; text-decoration: underline; font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; font-size: 16px;\">miRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; text-decoration: underline; font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; font-size: 16px;\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">, and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F10.html\" target=\"_blank\" style=\"background-color: transparent; color: rgb(70, 123, 150); transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; text-decoration: underline; font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; font-size: 16px;\">custom RNA synthesis\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\"> services. We can design and synthesize RNA Oligos of different lengths, different forms, and with various modifications according to customer’s needs, and also synthesize RNA sequences designed by customers. The RNA products we deliver are all purified by HPLC. ISO 9001-certified facilities and comprehensive quality control reports ensure the delivery of high-quality products.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-07 00:00:00","RNA Synthesis & Modifications—How to assess the silencing effect of siRNA","Assessing the silencing effect of siRNA is crucial to confirm its effectiveness in downregulating target gene expression. Several methods can be used, such as qPCR, Western Blot, ELISA, RNA-seq etc.","RNA Oligo, siRNA, Antisense Oligo, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:34:42","77,72,70,67,65",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":135,"categoryName":289,"title":323,"imgUrl":324,"content":325,"excerpt":78,"publishDate":326,"seoTitle":327,"seoDesc":328,"seoKeywords":319,"createDate":329,"sort":52,"relatedPostIds":330,"firstServiceProductId":49,"secondServiceProductIds":331,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"What is the difference between siRNA and sgRNA?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F12_20260630153901A113.png","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F12_20260630153907A114.png\" title=\"siRNA and sgRNA\" alt=\"siRNA and sgRNA\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA (small interfering RNA)\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\"> \u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA (single guide RNA)\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\"> \u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">are both short RNA molecules involved in RNA-mediated gene silencing, but they function in different RNA interference pathways and have distinct roles. Here are the key differences:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Ctable cellspacing=\"0\">\u003Ctbody style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctr class=\"firstRow\" style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Feature\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-width: 1px; border-style: solid; border-bottom-color: rgb(204, 204, 204); border-right-color: rgb(204, 204, 204); word-break: break-all;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">siRNA\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">sgRNA\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Function\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">RNA interference (gene silencing)\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">CRISPR-Cas9 genome editing\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Target\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">mRNA (post-transcriptionally)\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">DNA (for genetic modification)\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Structure\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">20-25 base pairs, duplex\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">100-120 nucleotides, single guide\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Origin\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Derived from long dsRNA or synthetic\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Engineered RNA, part of CRISPR-Cas9 system\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Purpose\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Gene silencing or knockdown\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Gene editing (DNA modification)\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Applications\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Functional genomics, therapeutics\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Genome editing, gene knockout\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Mechanism\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">mRNA degradation via RISC\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd valign=\"top\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid rgb(204, 204, 204); border-right: 1px solid rgb(204, 204, 204); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">DNA cleavage via Cas9 endonuclease\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003C\u002Ftbody>\u003C\u002Ftable>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In summary, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> is used for RNA-based gene silencing by targeting mRNA, while \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> is used in CRISPR-Cas9 systems for precise genome editing at the DNA level. Both are powerful tools but serve different purposes in molecular biology and gene therapy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F21.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">miRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F10.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">custom RNA synthesis services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">. We can design and synthesize RNA Oligos of different lengths, different forms, and with various modifications according to customer’s needs, and also synthesize RNA sequences designed by customers. The RNA products we deliver are all purified by HPLC. ISO 9001-certified facilities and comprehensive quality control reports ensure the delivery of high-quality products.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-09 00:00:00","RNA Synthesis & Modifications—What is the difference between siRNA and sgRNA","siRNA (small interfering RNA) and sgRNA (single guide RNA) are both short RNA molecules involved in RNA-mediated gene silencing, but they function in different RNA interference pathways and have distinct roles.","2025-04-30 16:35:23","77,70,69,68,67","13,58",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":139,"categoryName":289,"title":333,"imgUrl":334,"content":335,"excerpt":78,"publishDate":336,"seoTitle":337,"seoDesc":338,"seoKeywords":339,"createDate":340,"sort":84,"relatedPostIds":341,"firstServiceProductId":49,"secondServiceProductIds":311,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"What are the main applications of siRNA?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F13_20260630154005A115.png","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F13_20260630154013A116.png\" title=\"siRNA\" alt=\"siRNA\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA (small interfering RNA)\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\"> \u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">is a powerful tool for gene silencing, and it has a wide range of applications in both research and therapeutic contexts. Here are the key applications: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1. Gene Function Studies \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To study the function of specific genes by reducing their expression in cells or animals. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Knockdown of target genes to investigate their role in cellular processes such as cell cycle, apoptosis, or metabolism. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Creation of gene knockdowns for understanding disease mechanisms or identifying potential therapeutic targets. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. Functional Genomics \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To screen and identify genes involved in specific cellular pathways or disease processes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      High-throughput RNAi screening: Systematic knockdown of genes to identify those involved in processes like drug resistance, cancer progression, or immune response. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. Target Validation in Drug Development \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To validate potential drug targets by silencing specific genes and evaluating the resulting phenotypic changes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Use in early-stage drug discovery to identify and validate targets for new therapeutics, particularly in areas such as cancer, infectious diseases, and neurodegenerative diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Cancer Therapy \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To selectively silence genes involved in tumor growth, metastasis, or drug resistance. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Targeting oncogenes or genes that promote cancer cell survival or metastasis, thereby inhibiting tumor growth. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Sensitizing cancer cells to chemotherapy or targeted therapies by silencing drug resistance genes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. Viral Infections \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To reduce viral replication by silencing viral genes or host cell factors required for virus entry, replication, or assembly. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Developing antiviral therapies by targeting viral RNA, such as for HIV, influenza, hepatitis B, and other viral infections. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6. Neurological Disorders \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To treat diseases with genetic mutations, like Huntington's disease, by silencing the expression of toxic proteins. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●     Targeting RNA transcripts that encode disease-causing proteins (e.g., silencing mutant huntingtin in Huntington’s disease) or modulating genes related to neurodegeneration. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7. Gene Silencing in Model Organisms \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To create functional knockdowns in organisms such as mice, rats, or zebrafish for research purposes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: osiRNA is used to knock down genes in in vivo models to study gene function and disease progression, helping to model human diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8. Disease Treatment (Therapeutics) \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To develop siRNA-based therapeutics aimed at silencing disease-causing genes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Gene therapy: siRNA-based drugs are being developed to treat genetic disorders caused by overexpression of disease-causing genes (e.g., spinal muscular atrophy, acute liver diseases). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Ongoing clinical trials are exploring the use of siRNA for conditions like cancer, neurological disorders, and inherited genetic diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9. Precision Medicine \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To develop personalized therapies by silencing specific genes involved in individual patients' diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●      Using siRNA to target genes based on the genetic profile of patients, offering more tailored and effective treatment options. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10. Immune System Modulation \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To manipulate immune responses by silencing immune-related genes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●     Modulating immune checkpoints, cytokine production, or T-cell activation for autoimmune diseases or immunotherapy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●     Enhancing the immune response against infections or cancer by silencing suppressive pathways. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">11. Epigenetic Regulation \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Purpose: To investigate the role of epigenetic regulators in gene expression by silencing genes involved in chromatin modification or DNA methylation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●     Targeting epigenetic modifiers such as histone deacetylases (HDACs) or DNA methyltransferases (DNMTs) to study their effect on gene expression and cellular behavior. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In conclusion, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> has a broad range of applications across basic research, drug development, and therapeutic interventions, particularly in gene silencing, cancer treatment, viral infections, and genetic disorders. It is also being explored for its potential in precision medicine and immune system modulation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F21.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">miRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F10.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">custom RNA synthesis services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">. We can design and synthesize RNA Oligos of different lengths, different forms, and with various modifications according to customer’s needs, and also synthesize RNA sequences designed by customers. The RNA products we deliver are all purified by HPLC. ISO 9001-certified facilities and comprehensive quality control reports ensure the delivery of high-quality products.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-14 00:00:00","RNA Synthesis & Modifications—Applications of siRNA","siRNA has a broad range of applications across basic research, drug development, and therapeutic interventions, particularly in gene silencing, cancer treatment, viral infections, and genetic disorders. It is also being explored for its potential in precision medicine and immune system modulation.","RNA Oligo, siRNA, miRNA, Antisense Oligo, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:36:50","77,72,70,69,65",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":143,"categoryName":289,"title":343,"imgUrl":344,"content":345,"excerpt":78,"publishDate":346,"seoTitle":347,"seoDesc":348,"seoKeywords":349,"createDate":350,"sort":21,"relatedPostIds":351,"firstServiceProductId":49,"secondServiceProductIds":352,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"What are the main applications of miRNA?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F14_20260630154221A117.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F14_20260630154226A118.png\" title=\"miRNA\" alt=\"miRNA\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">miRNA (microRNA) is a class of small non-coding RNA molecules that play a critical role in regulating gene expression. Due to its functions and characteristics, miRNA has been widely applied in various fields. Below are its main applications: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1. Gene Function Research \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Regulatory Studies: Analyzing the interaction between miRNA and target genes helps uncover gene regulatory networks and their roles in processes like cell differentiation, proliferation, and apoptosis. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Disease Mechanism Studies: Investigating miRNA dysregulation provides insights into the molecular mechanisms of diseases such as cancer, metabolic disorders, and cardiovascular conditions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. Disease Diagnosis \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Biomarkers: miRNAs are considered potential biomarkers for detecting various diseases due to their tissue-specific expression and high stability. For example, specific miRNAs in blood or urine can be used for early cancer or diabetes diagnosis. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Disease Classification: Unique miRNA expression profiles can assist in disease subtyping and classification. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. Drug Development \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Targeted Therapy: Synthesized miRNA mimics or inhibitors can modulate disease-related miRNA expression, providing therapeutic approaches for conditions like cancer and viral infections. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Drug Sensitivity: miRNA expression levels may influence cellular responses to drugs, aiding in personalized treatment strategies. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Clinical Therapy \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      miRNA-Based Therapy: Therapies directly regulating miRNA expression, such as miRNA-delivering gene therapy, are being explored in clinical trials for cancer and genetic diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Regenerative Medicine: miRNAs can promote stem cell differentiation or regulate tissue repair, offering treatment potential for tissue injuries and degenerative diseases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. Agricultural Applications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Crop Improvement: Regulating miRNA expression can enhance plant stress resistance (e.g., against diseases or drought) and yield. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Genetic Breeding: miRNA markers are used for screening and improving desirable traits in crops. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6. Environmental and Toxicology Applications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Environmental Monitoring: Certain miRNAs respond sensitively to pollutants (e.g., heavy metals or chemical toxins), serving as early warning indicators of environmental pollution. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Toxicity Assessment: Changes in miRNA expression under toxic stress can be used to evaluate the potential hazards of chemicals. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7. Tool Development \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Research Tools: miRNA inhibitors, fluorescent probes, and microarrays are widely used for basic and applied research. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Bioinformatics: Computational tools for predicting miRNA-target relationships guide experimental designs and analyses. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In summary, miRNAs have broad prospects in life sciences and biotechnology. They play a significant role in disease diagnosis and treatment, agricultural improvement, and environmental monitoring, gaining increasing attention in these fields.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-21 00:00:00","RNA Synthesis & Modifications—Main applications of miRNA","miRNA (microRNA) is a class of small non-coding RNA molecules that play a critical role in regulating gene expression. They play a significant role in disease diagnosis and treatment, agricultural improvement, and environmental monitoring, gaining increasing attention in these fields.","RNA Oligo, siRNA, miRNA, microRNA, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:37:50","77,73,72,65,60","13,56",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":49,"title":81,"excerpt":354,"content":355,"seoTitle":356,"seoDesc":357,"seoKeywords":358,"sort":359,"createDate":360,"secondColor":361,"showOrderPic":5,"homeState":49,"homePic":362,"homeTitle":363,"homeDesc":364,"homeSort":359,"orderDesc":365,"orderUrl":366,"email":284,"phone":285,"onlineFormUrl":366,"serviceFeatureList":8,"productServiceSubList":8},"GenCefe Biotech provides high-quality siRNA , miRNA , sgRNA , and custom RNA synthesis and modification. We can design and synthesize RNA of different lengths, different forms, and with various modifications.","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fgencefebio.com\u002FproductsAndservices\u002F1-RNAi_Products_RNA_Synthesis.html\" style=\"font-family: Montserrat; color: rgb(0, 112, 192);\">\u003Cstrong>RNA synthesis\u003C\u002Fstrong>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> services, including siRNA, miRNA, sgRNA, and custom RNA oligonucleotide synthesis. We support both customer-designed sequences and custom RNA projects with flexible options for sequence length, modifications, and synthesis specifications. Production scales range from nmol and μmol quantities to larger-scale manufacturing, supporting diverse research and diagnostic development needs. Supported by ISO 9001 and ISO 13485 certified quality management systems, GenCefe delivers reliable RNA products, comprehensive QC documentation, and consistent project support.\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px; color: rgb(0, 0, 0);\">\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255); line-height: 1.75em;\">\u003Cspan style=\"font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box; font-family: Montserrat; font-size: 18px; color: rgb(54, 96, 146);\">RNA Synthesis Service Specifications:\u003C\u002Fspan>\u003Cspan style=\"color: rgb(79, 129, 189);\">\u003Cspan style=\"font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box; font-family: Montserrat; font-size: 18px;\">\u003C\u002Fspan>\u003Cspan style=\"font-size: 16px; font-family: Montserrat; font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box;\">\u003C\u002Fspan>\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat; font-size: 16px; color: rgb(0, 0, 0);\">\u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">The following table summarizes our RNA synthesis service options, specifications, and ordering details:\u003C\u002Fspan>\u003C\u002Fp>\u003Ctable>\u003Ctbody style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctr class=\"firstRow\" style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Product Name\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Length\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Amount\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Purification Method\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Turnaround Time (days)*\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Order\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">RNA oligo\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">nmol to μmol\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" rowspan=\"3\" colspan=\"1\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">HPLC\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">4-20\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" rowspan=\"3\" colspan=\"1\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-width: 1px; border-style: solid; border-bottom-color: rgb(0, 0, 0); border-right-color: rgb(0, 0, 0); word-break: break-all;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Please fill out the \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM\" target=\"_blank\" style=\"background-color: transparent; text-decoration: underline; transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">oligo order form\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">  and send it via email to \u003C\u002Fspan>\u003Ca href=\"mailto:oligo@gencefebio.com\" target=\"_blank\" style=\"background-color: transparent; text-decoration: underline; transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">oligo@gencefebio.com\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">.\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Modified\u002FLabeled RNA\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">nmol, to μmol\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">4-20\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Large-scale RNA Synthesis \u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">OD, nmol,\u003Cbr\u002F>milligram, gram\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Ca href=\"mailto:oligo@gencefebio.com\" target=\"_blank\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Get a Quote\u003C\u002Fspan>\u003C\u002Fa>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003C\u002Ftbody>\u003C\u002Ftable>\u003Cp>\u003Cem style=\"color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px;\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">* Turnaround time refers to the estimated time required for order processing, production, and shipment. Actual delivery times may vary depending on the destination and courier transit time.\u003C\u002Fspan>\u003C\u002Fem>\u003C\u002Fp>","RNA Synthesis | Fast Delivery & 50K+ siRNA Sets-GenCefe","GenCefe provides siRNA, miRNA, sgRNA, pegRNA, and custom RNA synthesis and modification services for RNAi, gene editing, and gene regulation.","RNA Synthesis, custom RNA oligo, RNA oligo synthesis, siRNA, miRNA, sgRNA, Antisense oligo",88,"2024-10-24 14:13:06","#48bfea","https:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2025\u002F04\u002F20\u002FRNA合成_蓝_20250420143026A002.png","RNA Synthesis","Custom synthesis and modification. 50,000+ pre-designed siRNA sets","Advance your RNA research with flexible RNA synthesis services, expert support, and dependable project execution.","https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM",[368,369,370,371,372,373,374],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":84,"parentId":49,"title":85,"subTitle":86,"excerpt":87,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":89,"parentId":49,"title":90,"subTitle":91,"excerpt":92,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":49,"newState":5,"type":93,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":95,"parentId":49,"title":96,"subTitle":97,"excerpt":98,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":100,"parentId":49,"title":101,"subTitle":102,"excerpt":103,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":105,"parentId":49,"title":106,"subTitle":107,"excerpt":108,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":49,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":31,"parentId":49,"title":110,"subTitle":78,"excerpt":111,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":113,"parentId":49,"title":114,"subTitle":78,"excerpt":115,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},[376,377],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":84,"parentId":49,"title":85,"subTitle":86,"excerpt":87,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":31,"parentId":49,"title":110,"subTitle":78,"excerpt":111,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":50,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8}]