[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"BasicInfo":3,"ApplicationsList":17,"BlogDetail60Info":51,"ProductServiceList":177},{"msg":4,"code":5,"data":6},"操作成功",0,{"companyName":7,"shortCompanyName":8,"title":8,"subTitle":9,"siteUrl":10,"siteDescription":11,"siteKeywords":12,"beiAnInfo":13,"email":14,"phone":15,"address":16},"GENCEFE Biotech (Wuxi, China) ","GenCefe","Your Reliable Partner of Oligo, Gene, Peptide, Protein ","https:\u002F\u002Fwww.gencefebio.com\u002F","GenCefe specializes in DNA, RNA, gene, peptide, and protein synthesis. Combined with our advanced AI-assisted design platform, we provide high-quality services for synthetic biology, molecular diagnostics, and drug discovery.","gene synthesis, oligo synthesis, RNA synthesis, DNA synthesis, peptide synthesis, protein expression and purification, gencefe, gencefe biotech","ICP备案：XXXXXXX\r\n苏公网安备32021402001785号","gene@gencefebio.com","+1 408-828-0438","17800 Castleton St, Ste 665, City of industry, CA 91748, USA",{"msg":4,"code":5,"data":18},[19,32,36,40,44,47],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":21,"title":22,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":23,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":24,"productService":20},null,9,"Oligonucleotide Drug Synthesis",90,[25,28],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":21,"orderNum":20,"ancestors":20,"id":26,"title":27,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":23,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},19,"Oligonucleotide Synthesis and Modifications",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":21,"orderNum":20,"ancestors":20,"id":29,"title":30,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":31,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},20,"Oligonucleotide biological evaluation platform",50,{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":33,"title":34,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":35,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},14,"Gene&Genome Editing",80,{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":37,"title":38,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":39,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},15,"Synthetic Biology",70,{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":41,"title":42,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":43,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},16,"Antibody Drug Discovery",60,{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":45,"title":46,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":31,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},17,"IVD Raw Material",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"parentName":20,"parentId":5,"orderNum":20,"ancestors":20,"id":48,"title":49,"excerpt":20,"content":20,"publishDate":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"productServiceId":20,"sort":50,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"applicationsSubList":20,"productService":20},18,"Vaccine and Novel Therapy",40,{"msg":4,"code":5,"data":52},{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":43,"categoryName":53,"title":54,"imgUrl":55,"content":56,"excerpt":57,"publishDate":58,"seoTitle":59,"seoDesc":60,"seoKeywords":61,"createDate":62,"sort":63,"relatedPostIds":64,"firstServiceProductId":65,"secondServiceProductIds":66,"relatedPost":67,"productService":125,"allSecondProductService":140,"twoSecondProductService":174},"RNA & Nucleic Acid Drug","What are the common types of modifications for small nucleic acids and their main functions?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F07_20260630144718A101.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F07_20260630144724A102.png\" title=\" RNA modifications, custom RNA synthesis\" alt=\" RNA modifications, custom RNA synthesis\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Various modifications are applied to small nucleic acids to enhance their stability, reduce immunogenicity, and improve targeting. Below are the most commonly used modifications and their primary functions: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Phosphorothioate (PS) Modification: Substitution of one of the non-bridging oxygen atoms in the phosphodiester backbone with sulfur. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Increases stability by protecting against nuclease degradation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    2'-O-Methyl (2'-OMe) Modification: Addition of a methyl group at the 2' position of the ribose sugar. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Enhances resistance to nuclease activity and reduces immune activation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Locked Nucleic Acid (LNA): Modification of the ribose sugar to "lock" the sugar in a particular conformation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Improves hybridization affinity and stability, especially for ASOs and miRNAs. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Fluorescent Labels: Attachment of fluorescent molecules to the oligonucleotide. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Used for tracking, imaging, and studying the distribution of nucleic acids. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Cholesterol Conjugation: Adding a cholesterol group to the oligonucleotide. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Enhances cellular uptake and promotes delivery across cell membranes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">   ●    Polyethylene Glycol (PEG) Conjugation: Attachment of PEG chains to the nucleic acid. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">          ●   Main Function: Increases solubility, reduces immunogenicity, and prolongs circulation time in vivo. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">These modifications are strategically used to optimize the pharmacokinetics, stability, and therapeutic efficacy of small nucleic acids.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F20.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">siRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F21.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">miRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">, \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F22.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">sgRNA\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, and \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FRNASynthesis\u002F10.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">custom RNA synthesis\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> services. We can design and synthesize RNA Oligos of different lengths, different forms, and with various modifications according to customer’s needs, and also synthesize RNA sequences designed by customers. The RNA products we deliver are all purified by HPLC. ISO 9001-certified facilities and comprehensive quality control reports ensure the delivery of high-quality products.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","","2024-12-24 00:00:00","RNA Synthesis & Modifications—RNA modifications and their functions","Various modifications are applied to small nucleic acids to enhance their stability, reduce immunogenicity, and improve targeting, such as PS, 2'-OMe, LNA, etc.","RNA Oligo, siRNA, miRNA, Antisense Oligo, RNA modifications, custom RNA synthesis","2025-04-28 17:06:54",7,"78,75,73,70,68",1,"13,60",[68,80,89,100,112],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":69,"categoryName":53,"title":70,"imgUrl":71,"content":72,"excerpt":57,"publishDate":73,"seoTitle":74,"seoDesc":75,"seoKeywords":76,"createDate":77,"sort":37,"relatedPostIds":78,"firstServiceProductId":65,"secondServiceProductIds":79,"relatedPost":20,"productService":20,"allSecondProductService":20,"twoSecondProductService":20},68,"What are the functions of miRNA inhibitors and mimics in microRNA research?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F15_20260630154315A119.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F15_20260630154322A120.png\" title=\"miRNA inhibitors and mimics\" alt=\"miRNA inhibitors and mimics\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In microRNA (miRNA) research, inhibitors and mimics are essential tools used to study the biological roles of specific miRNAs by either suppressing or mimicking their functions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Functions of miRNA Inhibitors \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Function: Suppress the activity of endogenous miRNAs, blocking their regulatory effects on target genes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Mechanism: Antisense oligonucleotides complementary to the mature miRNA bind to the miRNA, preventing its incorporation into the RNA-induced silencing complex (RISC) or its interaction with target mRNAs. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Experimental Purpose: Used to study the consequences of miRNA suppression, particularly in conditions where the miRNA is overexpressed, to assess its role in gene regulation and biological processes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: Suitable for investigating miRNA-related mechanisms in diseases, identifying miRNA targets, and validating their biological effects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Functions of miRNA Mimics \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Function: Enhance or replicate the function of specific miRNAs to promote their regulatory effects on target genes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Mechanism: Structurally mimic mature miRNAs using double-stranded RNA molecules. Once introduced into cells, mimics are processed by cellular machinery and loaded into RISC to act like endogenous miRNAs. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Experimental Purpose: Analyze the impact of miRNA overexpression, validate target gene regulation, and explore the role of specific miRNAs in biological or pathological contexts. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Application: Useful in studying under-expressed miRNAs, exploring therapeutic applications, and confirming miRNA-mRNA interactions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Key Differences and Application \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">miRNA inhibitors are used to suppress endogenous miRNA activity, making them suitable for studying miRNA inhibition effects. miRNA mimics, on the other hand, are used to enhance or mimic miRNA functions, making them ideal for studying the effects of miRNA overexpression. Combining these tools provides a comprehensive approach to exploring miRNA functions and their roles in specific biological or disease-related processes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-01-23 00:00:00","RNA Synthesis & Modifications—Functions of miRNA inhibitors and mimics in microRNA research","In microRNA (miRNA) research, inhibitors and mimics are essential tools used to study the biological roles of specific miRNAs by either suppressing or mimicking their functions."," RNA Oligo, miRNA, microRNA, miRNA inhibitors, miRNA mimics, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:38:35","74,72,70,67,65","13,56",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":39,"categoryName":53,"title":81,"imgUrl":82,"content":83,"excerpt":57,"publishDate":84,"seoTitle":85,"seoDesc":86,"seoKeywords":76,"createDate":87,"sort":45,"relatedPostIds":88,"firstServiceProductId":65,"secondServiceProductIds":79,"relatedPost":20,"productService":20,"allSecondProductService":20,"twoSecondProductService":20},"What are the major challenges in miRNA therapeutics research?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F17_20260630154553A123.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F17_20260630154600A124.png\" title=\"major challenges\" alt=\"major challenges\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Despite progress, miRNA therapeutics faces several challenges that need to be addressed for clinical success. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.  Delivery Challenges \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Effective delivery to target tissues or cells without degradation remains a major hurdle. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      The lack of non-toxic and efficient delivery vehicles limits widespread clinical application. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.  Off-Target Effects \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      miRNAs regulate multiple genes and pathways. Altering their expression may cause unintended effects on non-target genes, leading to toxicity or adverse outcomes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.  Safety Concerns \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Synthetic miRNA molecules and delivery carriers can trigger immune responses or inflammation, posing challenges for long-term safety. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.  Complex Biological Roles \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      The functions of miRNAs can vary depending on the disease context, making therapeutic design and validation more difficult. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.  Regulatory and Economic Barriers \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Demonstrating long-term safety, efficacy, and consistent manufacturing processes is challenging under current regulatory frameworks. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      The high cost of development and scaling production can limit commercial viability. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">While miRNA therapeutics offer significant promise, challenges such as delivery efficiency, off-target effects, and regulatory hurdles remain obstacles to clinical success. Continued innovations in nanotechnology, precision medicine, and delivery systems will be critical for overcoming these barriers and fully unlocking the potential of miRNA-based treatments.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-02-18 00:00:00","RNA Synthesis & Modifications—Major challenges in miRNA therapeutics research","While miRNA therapeutics offer significant promise, challenges such as delivery efficiency, off-target effects, and regulatory hurdles remain obstacles to clinical success.","2025-04-30 16:43:05","73,72,71,69,64",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":90,"categoryName":53,"title":91,"imgUrl":92,"content":93,"excerpt":57,"publishDate":94,"seoTitle":95,"seoDesc":96,"seoKeywords":97,"createDate":98,"sort":29,"relatedPostIds":99,"firstServiceProductId":65,"secondServiceProductIds":66,"relatedPost":20,"productService":20,"allSecondProductService":20,"twoSecondProductService":20},73,"What is the current progress in antisense oligonucleotide (ASO) drug development? ","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F20_20260630155023A129.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F20_20260630155029A130.png\" title=\"antisense oligonucleotide (ASO) drug development\" alt=\"antisense oligonucleotide (ASO) drug development\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Antisense oligonucleotide (ASO) drugs have advanced significantly, establishing themselves as a versatile and effective platform for treating genetic and other complex diseases. Key progress includes the following: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1. Approved ASO Therapies \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Several ASO drugs have been approved for clinical use, addressing a wide range of conditions: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Nusinersen (Spinraza): The first FDA-approved ASO for spinal muscular atrophy (SMA), developed by Ionis Pharmaceuticals and Biogen. It targets the SMN2 gene to enhance functional SMN protein production. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Mipomersen (Kynamro): Used to treat familial hypercholesterolemia by reducing apolipoprotein B (ApoB) production. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Eteplirsen (Exondys 51): Targets exon skipping in the dystrophin gene for Duchenne muscular dystrophy (DMD). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. Expanding Disease Applications \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Neurological Diseases: ASOs have shown promise in treating Huntington’s disease (e.g., Tominersen) and amyotrophic lateral sclerosis (ALS). These drugs are designed to reduce harmful protein accumulation or correct splicing defects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Oncology: ASOs are being investigated to silence oncogenes or re-sensitize tumors to chemotherapy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Metabolic Disorders: Targeting liver-specific pathways, ASOs like inclisiran reduce low-density lipoprotein (LDL) cholesterol. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. Innovations in Chemistry \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Chemical modifications have improved ASO stability, delivery, and efficacy: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Phosphorothioate Backbones: Enhance stability and resistance to nucleases. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      2'-O-Methyl (2'-OMe) and 2'-Methoxyethyl (2'-MOE): Increase binding affinity and reduce toxicity. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Gapmers: ASOs designed to activate RNase H-mediated cleavage, improving potency. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Delivery Advancements \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Targeted Delivery: Liver-specific delivery through conjugates like N-acetylgalactosamine (GalNAc) has reduced off-target effects and improved safety profiles. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Intrathecal Administration: Direct delivery to the central nervous system (CNS) for neurological diseases, bypassing the blood-brain barrier. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. Ongoing Clinical Trials \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Dozens of ASO drugs are in clinical development, targeting diseases such as Alzheimer’s, cancer, and rare genetic conditions. Emerging therapies include RNA-targeting ASOs for exon skipping, splicing correction, and gene silencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">ASO drug development has achieved remarkable progress, with approved therapies addressing rare genetic diseases and expanding into more prevalent conditions like cancer and neurological disorders. Advances in chemistry, delivery systems, and disease-specific targeting are driving the growth of ASOs as a transformative therapeutic platform.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-02-27 00:00:00","RNA Synthesis & Modifications—Progress in antisense oligonucleotide (ASO) drug development"," ASO drug development has achieved remarkable progress, with approved therapies addressing rare genetic diseases and expanding into more prevalent conditions like cancer and neurological disorders.","RNA Oligo, ASO, antisense oligo, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:50:17","67,66,65,64,63",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":101,"categoryName":53,"title":102,"imgUrl":103,"content":104,"excerpt":57,"publishDate":105,"seoTitle":106,"seoDesc":107,"seoKeywords":108,"createDate":109,"sort":110,"relatedPostIds":111,"firstServiceProductId":65,"secondServiceProductIds":66,"relatedPost":20,"productService":20,"allSecondProductService":20,"twoSecondProductService":20},75,"What are the ASO pipelines currently in clinical stages? ","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F21_20260630155158A131.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F21_20260630155203A132.png\" title=\"ASO pipelines\" alt=\"ASO pipelines\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Antisense oligonucleotide (ASO) drugs have shown significant progress in clinical development, targeting a wide range of diseases, from rare genetic disorders to more common conditions. Since the approval of the first ASO drug, Fomivirsen, in 1998, the pipeline has expanded substantially. Here are some key ASO drugs currently in clinical stages: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Approved ASO Drugs \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Nusinersen (Spinraza): Approved in 2016 by the FDA for treating spinal muscular atrophy (SMA). It enhances the production of functional SMN protein. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Eteplirsen: Approved for Duchenne muscular dystrophy (DMD), it promotes exon skipping to restore functional dystrophin protein. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Inotersen: Approved for hereditary transthyretin-mediated amyloidosis (hATTR), it reduces transthyretin protein levels to slow disease progression. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">ASO Drugs in Clinical Trials \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Tominersen: Designed for Huntington’s disease, it reduces mutant huntingtin protein levels. Currently in Phase II clinical trials. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      ON363: Targets FUS-related amyotrophic lateral sclerosis (ALS) by decreasing FUS protein production. It has reached Phase III clinical trials. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      ALG-020572: A novel ASO for chronic hepatitis B (CHB) treatment, currently in Phase I trials. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      IONIS-TTR-LRx: A liver-targeting ASO for hATTR amyloidosis, improving delivery efficiency with GalNAc conjugation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Global and Regional Progress\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">ASO development is advancing globally, with contributions from leading biotechnology companies and institutions. In China, domestic enterprises such as Hengrui Medicine are developing ASO drugs, with 16 oligonucleotide-based candidates approved for clinical studies, marking a significant milestone in local innovation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">The ASO clinical pipeline has grown to address diverse diseases, including neurodegenerative disorders, rare genetic diseases, and viral infections. Advances in chemical modifications, delivery systems, and disease-specific targeting continue to drive the evolution of ASO therapeutics. These developments promise to bring more ASO drugs to market, benefiting patients worldwide.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-03-06 00:00:00","RNA Synthesis & Modifications—ASO pipelines currently in clinical stages","The ASO clinical pipeline has grown to address diverse diseases, including neurodegenerative disorders, rare genetic diseases, and viral infections.","RNA Oligo, ASO, antisense oligo, RNAi, RNA interference, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:51:38",22,"71,67,64,60",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":113,"categoryName":53,"title":114,"imgUrl":115,"content":116,"excerpt":57,"publishDate":117,"seoTitle":118,"seoDesc":119,"seoKeywords":120,"createDate":121,"sort":122,"relatedPostIds":123,"firstServiceProductId":65,"secondServiceProductIds":124,"relatedPost":20,"productService":20,"allSecondProductService":20,"twoSecondProductService":20},78,"What are the considerations in sgRNA design?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F24_20260630155540A137.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F24_20260630155545A138.png\" title=\"sgRNA design\" alt=\"sgRNA design\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Designing an effective single-guide RNA (sgRNA) is crucial for the success of CRISPR-based genome editing. Poorly designed sgRNAs can result in low editing efficiency or unintended off-target effects. To optimize sgRNA performance, the following factors should be considered: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1. Target Sequence Selection \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      PAM Sequence: Ensure the presence of a protospacer adjacent motif (PAM) specific to the Cas protein being used (e.g., NGG for SpCas9). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      On-Target Specificity: Select sequences unique to the target gene to avoid unintended binding and cleavage at off-target sites. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2. sgRNA Efficiency \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      GC Content: Aim for a GC content of 40–60% to ensure stable binding to the target DNA while avoiding excessive secondary structure formation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Positioning: For coding regions, design sgRNAs near the start codon to maximize functional disruption. For regulatory regions, choose sgRNAs close to critical transcription factor binding sites. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Avoidance of Secondary Structures: Use bioinformatics tools to predict and minimize hairpin formations within the sgRNA sequence. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3. Off-Target Minimization \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Mismatch Tolerance: Avoid sgRNAs with high similarity to non-target regions, especially near the seed region (6–12 nucleotides proximal to the PAM). \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Bioinformatics Screening: Utilize tools like CRISPRoff, CCTop, or Benchling to predict and rank potential off-target effects. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4. Delivery Compatibility \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Cas Protein Compatibility: Design sgRNAs specific to the Cas variant (e.g., Cas9, Cas12a) being used. Cas12a sgRNAs are typically longer than those for Cas9. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Vector Requirements: Ensure sgRNA sequences fit within the constraints of the delivery vector, such as plasmids, AAV, or LNPs. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5. Functional and Experimental Context \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Gene Function: For gene knockouts, target exons shared across all transcript isoforms. For precise edits, consider proximity to the intended base-editing site. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Multiplex Editing: When designing sgRNAs for multiplexing, ensure compatibility to avoid competition for Cas proteins. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">     ●      Validation: Always include controls, such as scrambled or non-targeting sgRNAs, to confirm specificity and efficacy.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Effective sgRNA design is a balance between on-target efficiency, minimal off-target activity, and compatibility with the experimental context. Leveraging computational tools and iterative testing can significantly enhance the reliability of CRISPR-based experiments. Proper design is critical for achieving precise and effective genome editing in both research and therapeutic applications.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-03-18 00:00:00","RNA Synthesis & Modifications—What are the considerations in sgRNA design?","Effective sgRNA design is a balance between on-target efficiency, minimal off-target activity, and compatibility with the experimental context.","RNA Oligo, sgRNA, guide RNA, gene editing, CRISPR, sgRNA design, Small nucleic acid drug, custom RNA synthesis","2025-04-30 16:53:37",25,"75,73,70,65,56","13,58",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":65,"title":126,"excerpt":127,"content":128,"seoTitle":129,"seoDesc":130,"seoKeywords":131,"sort":132,"createDate":133,"secondColor":134,"showOrderPic":5,"homeState":65,"homePic":135,"homeTitle":136,"homeDesc":137,"homeSort":132,"orderDesc":138,"orderUrl":139,"email":14,"phone":15,"onlineFormUrl":139,"serviceFeatureList":20,"productServiceSubList":20},"RNAi Products & RNA Synthesis","GenCefe Biotech provides high-quality siRNA , miRNA , sgRNA , and custom RNA synthesis and modification. We can design and synthesize RNA of different lengths, different forms, and with various modifications.","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech provides high-quality \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fgencefebio.com\u002FproductsAndservices\u002F1-RNAi_Products_RNA_Synthesis.html\" style=\"font-family: Montserrat; color: rgb(0, 112, 192);\">\u003Cstrong>RNA synthesis\u003C\u002Fstrong>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> services, including siRNA, miRNA, sgRNA, and custom RNA oligonucleotide synthesis. We support both customer-designed sequences and custom RNA projects with flexible options for sequence length, modifications, and synthesis specifications. Production scales range from nmol and μmol quantities to larger-scale manufacturing, supporting diverse research and diagnostic development needs. Supported by ISO 9001 and ISO 13485 certified quality management systems, GenCefe delivers reliable RNA products, comprehensive QC documentation, and consistent project support.\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px; color: rgb(0, 0, 0);\">\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp style=\"padding: 0px; margin-top: 10px; margin-bottom: 10px; box-sizing: border-box; color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px; text-wrap-mode: wrap; background-color: rgb(255, 255, 255); line-height: 1.75em;\">\u003Cspan style=\"font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box; font-family: Montserrat; font-size: 18px; color: rgb(54, 96, 146);\">RNA Synthesis Service Specifications:\u003C\u002Fspan>\u003Cspan style=\"color: rgb(79, 129, 189);\">\u003Cspan style=\"font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box; font-family: Montserrat; font-size: 18px;\">\u003C\u002Fspan>\u003Cspan style=\"font-size: 16px; font-family: Montserrat; font-weight: bolder; padding: 0px; margin: 0px; box-sizing: border-box;\">\u003C\u002Fspan>\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat; font-size: 16px; color: rgb(0, 0, 0);\">\u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">The following table summarizes our RNA synthesis service options, specifications, and ordering details:\u003C\u002Fspan>\u003C\u002Fp>\u003Ctable>\u003Ctbody style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctr class=\"firstRow\" style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Product Name\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Length\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Amount\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Purification Method\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Turnaround Time (days)*\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  background-color: rgb(31, 73, 125); border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(255, 255, 255); font-family: Montserrat; font-size: 16px;\">Order\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">RNA oligo\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">nmol to μmol\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" rowspan=\"3\" colspan=\"1\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">HPLC\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">4-20\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" rowspan=\"3\" colspan=\"1\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-width: 1px; border-style: solid; border-bottom-color: rgb(0, 0, 0); border-right-color: rgb(0, 0, 0); word-break: break-all;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Please fill out the \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM\" target=\"_blank\" style=\"background-color: transparent; text-decoration: underline; transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">oligo order form\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">  and send it via email to \u003C\u002Fspan>\u003Ca href=\"mailto:oligo@gencefebio.com\" target=\"_blank\" style=\"background-color: transparent; text-decoration: underline; transition: 0.5s; padding: 0px; margin: 0px; box-sizing: border-box; color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">\u003Cspan style=\"color: rgb(28, 72, 127); font-family: Montserrat; font-size: 16px;\">oligo@gencefebio.com\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">.\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Modified\u002FLabeled RNA\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">nmol, to μmol\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">4-20\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003Ctr style=\"padding: 0px; margin: 0px; box-sizing: border-box;\">\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Large-scale RNA Synthesis \u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">5-100 nt\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">OD, nmol,\u003Cbr\u002F>milligram, gram\u003Cbr\u002F>level\u003C\u002Fspan>\u003C\u002Fp>\u003C\u002Ftd>\u003Ctd width=\"288\" valign=\"middle\" align=\"center\" style=\"padding: 3px 5px; margin: 0px; box-sizing: border-box; border-bottom: 1px solid #000; border-right: 1px solid #000;  border-top-width: 1px; border-left-width: 1px; border-top-style: solid; border-left-style: solid;\">\u003Cp>\u003Ca href=\"mailto:oligo@gencefebio.com\" target=\"_blank\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">Get a Quote\u003C\u002Fspan>\u003C\u002Fa>\u003C\u002Fp>\u003C\u002Ftd>\u003C\u002Ftr>\u003C\u002Ftbody>\u003C\u002Ftable>\u003Cp>\u003Cem style=\"color: rgb(68, 68, 68); font-family: "Helvetica Neue", Helvetica, Arial, -apple-system, system-ui, sans-serif; font-size: 14px;\">\u003Cspan style=\"font-family: Montserrat; font-size: 16px;\">* Turnaround time refers to the estimated time required for order processing, production, and shipment. Actual delivery times may vary depending on the destination and courier transit time.\u003C\u002Fspan>\u003C\u002Fem>\u003C\u002Fp>","RNA Synthesis | Fast Delivery & 50K+ siRNA Sets-GenCefe","GenCefe provides siRNA, miRNA, sgRNA, pegRNA, and custom RNA synthesis and modification services for RNAi, gene editing, and gene regulation.","RNA Synthesis, custom RNA oligo, RNA oligo synthesis, siRNA, miRNA, sgRNA, Antisense oligo",88,"2024-10-24 14:13:06","#48bfea","https:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2025\u002F04\u002F20\u002FRNA合成_蓝_20250420143026A002.png","RNA Synthesis","Custom synthesis and modification. 50,000+ pre-designed siRNA sets","Advance your RNA research with flexible RNA synthesis services, expert support, and dependable project execution.","https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM",[141,147,152,157,162,167,170],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":142,"parentId":65,"title":143,"subTitle":144,"excerpt":145,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},13,"Custom siRNA Synthesis","Custom siRNA Synthesis and Modifications for Diverse Research Needs","GenCefe provides siRNA design and custom synthesis, including standard siRNA oligos and modified siRNA oligos, to meet customers'specific needs.","服务",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":110,"parentId":65,"title":148,"subTitle":149,"excerpt":150,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":65,"newState":5,"type":151,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},"Pre-Designed siRNA Sets","Ready-to-Order siRNA Sets for Targeted Gene-Silencing Research","GenCefe Biotech provides 50,000+ pre-designed siRNA sets for human, rat, and mouse. Guaranteed gene silencing, free control siRNA.","产品",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":153,"parentId":65,"title":154,"subTitle":155,"excerpt":156,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},56,"Custom miRNA Mimic\u002FInhibitor","Custom miRNA Mimics and Inhibitors for In Vitro and In Vivo Research","GenCefe has developed various miRNA mimics for gain-of-function studies and inhibitors for loss-of-function studies, with different options for both in vitro and in vivo experiments.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":158,"parentId":65,"title":159,"subTitle":160,"excerpt":161,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},58,"sgRNA Synthesis","Custom gRNA Synthesis and Design with Flexible Options and Quality Control","GenCefe provides sgRNA design and chemical synthesis services. Deliverables are tested by mass spectrometry for sequence accuracy and purified by HPLC.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":163,"parentId":65,"title":164,"subTitle":165,"excerpt":166,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":65,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},59,"circRNA","Flexible circRNA Synthesis with Chemical and IVT Platforms.","GenCefe Biotech offers both chemical and IVT synthesis of circRNA, enabling customized solutions with flexible lengths and purity to meet diverse research needs.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":43,"parentId":65,"title":168,"subTitle":57,"excerpt":169,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},"RNA Modifications","GenCefe Biotech provides comprehensive RNA modification, labeling, and conjugation services to meet the needs of different types of nucleic acid drug delivery.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":171,"parentId":65,"title":172,"subTitle":57,"excerpt":173,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},61,"RNA QC Standards","GenCefe Biotech is committed to providing high-quality oligonucleotide products and services; we perform HPLC detection and provide COA files for all of our oligonucleotide products by default. ",[175,176],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":142,"parentId":65,"title":143,"subTitle":144,"excerpt":145,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":43,"parentId":65,"title":168,"subTitle":57,"excerpt":169,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},{"msg":4,"code":5,"data":178},[179,215,224,265,276,291,300,324,352,376],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":21,"title":180,"excerpt":20,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"sort":20,"createDate":20,"secondColor":20,"showOrderPic":20,"homeState":20,"homePic":20,"homeTitle":20,"homeDesc":20,"homeSort":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"serviceFeatureList":20,"productServiceSubList":181},"Gene Synthesis and Related Services",[182,187,192,197,202,206,211],{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":183,"parentId":21,"title":184,"subTitle":185,"excerpt":186,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":65,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},11,"Gene Synthesis","Custom gene synthesis solutions with optimized design and reliable quality.","GenCefe gene synthesis services - professional technical support, free codon optimization, fast delivery and 100% accuracy guarantee.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":188,"parentId":21,"title":189,"subTitle":190,"excerpt":191,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},12,"Codon Optimization","Host-specific codon optimization to support efficient recombinant protein expression.","GenCefe has introduced advanced AI technology into codon optimization and independently developed the Codon Optimization Tool, which can significantly increase the protein expression level and success rate.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":193,"parentId":21,"title":194,"subTitle":195,"excerpt":196,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},48,"Gene Fragments","Custom DNA fragments for flexible downstream assembly.","GenCefe offers DNA fragment synthesis service with strict QC standards and fast turnaround, saving time and budget for you.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":198,"parentId":21,"title":199,"subTitle":200,"excerpt":201,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},49,"Subcloning & PCR Cloning","Custom Cloning Solutions for Tailored Plasmid Construction","GenCefe Biotech can help customers clone the target gene into any site of the specified vector to meet different needs.",{"createBy":20,"createTime":20,"updateBy":20,"updateTime":20,"remark":20,"id":31,"parentId":21,"title":203,"subTitle":204,"excerpt":205,"content":20,"seoTitle":20,"seoDesc":20,"seoKeywords":20,"createDate":20,"orderDesc":20,"orderUrl":20,"email":20,"phone":20,"onlineFormUrl":20,"sort":20,"hotState":5,"newState":5,"type":146,"customerTestimonials":20,"productCatalog":20,"relatedTitles":20,"productService":20,"customerTestimonialsList":20,"relatedTitlesList":20,"serviceFeatureSubList":20,"productCatalogList":20},"Plasmid Preparation","Flexible plasmid preparation tailored to your specific needs.","GenCefe provides high-quality plasmid preparation from micrograms to grams, with strict QC for batch 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