[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"BlogDetail49Info":3,"ApplicationsList":145,"BasicInfo":176,"ProductServiceList":187},{"msg":4,"code":5,"data":6},"操作成功",0,{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":8,"categoryName":9,"title":10,"imgUrl":11,"content":12,"excerpt":13,"publishDate":14,"seoTitle":15,"seoDesc":16,"seoKeywords":17,"createDate":18,"sort":19,"relatedPostIds":20,"firstServiceProductId":21,"secondServiceProductIds":22,"relatedPost":23,"productService":84,"allSecondProductService":101,"twoSecondProductService":142},null,49,"Primers & Probes","What are the commonly used fluorescent modification groups?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630142611A080.jpg","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630142618A081.jpg\" title=\"fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification\" alt=\"fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">Oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;(oligos) are frequently modified with various fluorescent groups to enable detection, imaging, and analysis of nucleic acids. Some common fluorescent modifications used in oligos include:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Fluorescein (FAM): FAM is a widely used fluorescent dye that emits green fluorescence when excited by light of appropriate wavelength. It is commonly employed in applications such as real-time PCR, DNA sequencing, and fluorescence in situ hybridization (FISH).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Tetramethylrhodamine (TAMRA): TAMRA is a red fluorescent dye often used as a quencher in oligonucleotide probes. It can quench the fluorescence of nearby fluorophores via fluorescence resonance energy transfer (FRET), making it useful for probe-based assays such as qPCR and molecular beacon assays.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Cyanine Dyes (Cy3, Cy5, Cy5.5, Cy7): Cyanine dyes are a family of fluorescent dyes with varying emission wavelengths. Cy3 emits green fluorescence, Cy5 emits red fluorescence, and Cy5.5 and Cy7 emit near-infrared fluorescence. They are commonly used in multiplexed assays, microarray experiments, and fluorescence imaging techniques.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.Alexa Fluor Dyes: Alexa Fluor dyes are a series of highly fluorescent dyes with a wide range of emission wavelengths. They are characterized by their photostability and brightness, making them suitable for various fluorescence-based applications such as immunofluorescence staining, flow cytometry, and microscopy.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Rhodamine Dyes (ROX, ROX): Rhodamine dyes emit red-orange fluorescence and are often used as reference dyes in real-time PCR and DNA sequencing reactions. They serve as internal standards for normalization of fluorescence signals, aiding in accurate quantification of target nucleic acids.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.BODIPY Dyes: BODIPY dyes are a family of fluorescent dyes with high quantum yields and excellent photostability. They are used in various applications including fluorescence labeling, imaging, and detection of nucleic acids and proteins.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Quasar Dyes: Quasar dyes are a series of proprietary fluorescent dyes developed by Biosearch Technologies. They offer excellent brightness and photostability, making them suitable for applications such as SNP genotyping, FRET assays, and fluorescence in situ hybridization (FISH).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">These are just a few examples of the many fluorescent modifications available for&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\"> The choice of fluorescent dye depends on factors such as emission wavelength, compatibility with detection systems, and specific requirements of the experiment or assay.\u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe provides customized\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">&nbsp;\u003C\u002Fspan>\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">DNA oligo modification\u002Flabeling services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes\u002Fprimers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">modified primers\u002Fprobes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","","2024-07-23 00:00:00","Oligo Synthesis—Fluorescent Modifications","Oligos are frequently modified with various fluorescent groups to enable detection, imaging, and analysis of nucleic acids. Some common fluorescent modifications used in oligos include: FAM, TAMRA, Cyanine Dyes, etc. ","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification, Quasar570 modification, Cy3 Cy5 modification","2025-04-28 16:35:59",11,"43,42,41,40,39",2,"62,63",[24,37,49,60,72],{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":25,"categoryName":9,"title":26,"imgUrl":27,"content":28,"excerpt":13,"publishDate":29,"seoTitle":30,"seoDesc":31,"seoKeywords":32,"createDate":33,"sort":34,"relatedPostIds":35,"firstServiceProductId":21,"secondServiceProductIds":36,"relatedPost":7,"productService":7,"allSecondProductService":7,"twoSecondProductService":7},39,"What are the applications of Oligo in next-generation sequencing (NGS)?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F01_20260630134319A060.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F01_20260630134326A061.jpg\" title=\"probe synthesis, oligo modifications\" alt=\"probe synthesis, oligo modifications\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In next-generation sequencing (NGS),&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"font-family: Montserrat; text-decoration: underline;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">oligos\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;(short for oligonucleotides) have various applications, including:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1)&nbsp;&nbsp;Targeted Sequencing: Oligos are used to design primers for selective amplification of specific DNA or RNA sequences, enabling the study of particular genes, genomic regions, or transcripts.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2)&nbsp;&nbsp;Library Preparation: Oligos are employed to label DNA or RNA fragments during library preparation processes, facilitating library construction and enrichment.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3)&nbsp;&nbsp;Polymerase Chain Reaction (PCR): Oligos serve as primers in PCR to amplify target DNA segments, playing a crucial role in DNA replication and amplification.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4)&nbsp;&nbsp;RNA Capture and Sequencing: Oligos are utilized to capture RNA, such as reverse transcribing RNA and using specific oligos for library enrichment, enabling RNA sequencing analysis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5)&nbsp;&nbsp;Molecular Barcoding: Oligos can function as molecular barcodes, tagging samples for differentiation in high-throughput sequencing, and aiding in sample tracking and data analysis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6)&nbsp;&nbsp;Single Cell Sequencing: In single-cell sequencing, oligos are used to label RNA or DNA of individual cells, facilitating identification and differentiation during subsequent sequencing.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7)&nbsp;&nbsp;SNP Genotyping: Oligos are used for the detection and genotyping of single nucleotide polymorphisms (SNPs), applicable in genetic disease research, population genetics, among other fields.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8)&nbsp;&nbsp;DNA\u002FRNA Hybridization: Oligos are employed in hybridization experiments to bind with target DNA or RNA sequences, useful for detection, separation, and quantification of specific sequences.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9)&nbsp;&nbsp;DNA\u002FRNA Aptamers: Oligos are used for screening and constructing DNA or RNA aptamers, high-selectivity molecular probes capable of binding to specific molecules or proteins.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10)&nbsp;&nbsp;Epigenetic Analysis: Oligos are utilized for the enrichment and detection of methylation or histone modification sites, aiding in the study of epigenetic changes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech’s professional technical team utilizes our advanced&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"font-family: Montserrat; text-decoration: underline;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">oligo synthesis and modification\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;technology platform to provide adapters for NGS library construction, blockers and capture probes for hybridization capture, and primers for multiplex PCR. We are committed to supporting your NGS experiments with&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">high-quality primers and probes&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-03-29 00:00:00","Oligo Synthesis—Oligo applications in next-generation sequencing (NGS)","In next-generation sequencing (NGS), oligos (short for oligonucleotides) have various applications, including: Targeted Sequencing, Library Preparation, RNA Capture and Sequencing, Molecular Barcoding, etc.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, NGS primer, NGS probe","2025-04-28 16:18:12",1,"53,52,51,50,49","62,65",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":38,"categoryName":9,"title":39,"imgUrl":40,"content":41,"excerpt":13,"publishDate":42,"seoTitle":43,"seoDesc":44,"seoKeywords":45,"createDate":46,"sort":21,"relatedPostIds":47,"firstServiceProductId":21,"secondServiceProductIds":48,"relatedPost":7,"productService":7,"allSecondProductService":7,"twoSecondProductService":7},40,"What are the applications of Oligo in quantitative polymerase chain reaction (qPCR)?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F02_20260630135913A062.jpg","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F02_20260630135920A063.jpg\" title=\"probe synthesis, oligo modifications, primer synthesis\" alt=\"probe synthesis, oligo modifications, primer synthesis\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"font-family: Montserrat; text-decoration: underline;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">Oligos\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;have several applications in quantitative polymerase chain reaction (qPCR), including:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1)&nbsp;&nbsp;Primer Design: Oligos are crucial components in qPCR primer design. They are designed to anneal to specific regions flanking the target DNA sequence, enabling selective amplification during qPCR.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2)&nbsp;&nbsp;Probe Design: Oligos are used to design fluorescent probes for qPCR assays. These probes typically consist of a fluorescent dye and a quencher molecule, and they hybridize to the target DNA sequence during qPCR, allowing for real-time monitoring of amplification.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3)&nbsp;&nbsp;Multiplexing: Oligos enable the multiplexing of qPCR reactions, where multiple target sequences can be amplified simultaneously in a single reaction using different sets of primers and probes labeled with distinct fluorescent dyes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4)&nbsp;&nbsp;Detection of Gene Expression: Oligo-based qPCR assays are widely used for gene expression analysis. By designing oligo primers and probes specific to target genes, qPCR can quantitatively measure the expression levels of those genes in biological samples.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5)&nbsp;&nbsp;Allelic Discrimination: Oligos can be designed to distinguish between different alleles of a gene in qPCR assays, enabling the detection of single nucleotide polymorphisms (SNPs) or mutations associated with genetic diseases or traits.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6)&nbsp;&nbsp;Absolute Quantification: Oligo-based qPCR can be used for absolute quantification of target nucleic acids by comparing the amplification curves of unknown samples to standard curves generated from known concentrations of reference nucleic acids.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7)&nbsp;&nbsp;Pathogen Detection: Oligo-based qPCR assays are employed for the sensitive and specific detection of pathogens such as viruses, bacteria, and fungi in clinical samples, environmental samples, and food samples.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Overall,&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligos&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;play a crucial role in primer and probe design, enabling the specificity, sensitivity, and versatility of qPCR assays for various applications in molecular biology, diagnostics, and research.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech has established an advanced oligo synthesis and modification technology platform with an experienced technical team to provide customized qPCR primers and probes. We are committed to assisting your research and diagnostic applications with high accuracy and reliability of&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F209.html\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">qPCR primers and probes&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-04-06 00:00:00","Oligo Synthesis—Oligo applications in quantitative polymerase chain reaction (qPCR)","Oligos have several applications in quantitative polymerase chain reaction (qPCR), including: primer design, probe design, multiplexing, detection of gene expression, etc.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, qPCR primer, qPCR probe","2025-04-28 16:19:57","48,47,46,45,44","62,64",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":50,"categoryName":9,"title":51,"imgUrl":52,"content":53,"excerpt":13,"publishDate":42,"seoTitle":54,"seoDesc":55,"seoKeywords":56,"createDate":57,"sort":58,"relatedPostIds":59,"firstServiceProductId":21,"secondServiceProductIds":22,"relatedPost":7,"productService":7,"allSecondProductService":7,"twoSecondProductService":7},41,"What are the commonly used Oligo purification methods and which applications are they suitable for?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F1_20260702141111A197.png","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F1_20260702141150A199.png\" title=\"oligo modifications, primer synthesis\" alt=\"oligo modifications, primer synthesis\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F204.html\" style=\"font-family: Montserrat;\">\u003Cspan style=\"color: rgb(79, 129, 189);\">Oligos\u003C\u002Fspan>&nbsp;\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">can be purified using various methods, each with its own advantages and suitability for different applications:\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1)&nbsp;&nbsp;Desalting: Desalting is a simple purification method that removes small molecular weight impurities such as salts and unincorporated nucleotides. It is suitable for applications where high purity is not critical, such as some molecular biology applications like PCR amplification.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2)&nbsp;&nbsp;Solid Phase Extraction (SPE): SPE involves binding oligos to a solid support, followed by washing to remove impurities and elution of purified oligos. It offers higher purity compared to desalting and is suitable for applications requiring higher purity, such as sequencing, cloning, and PCR.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3)&nbsp;&nbsp;High Performance Liquid Chromatography (HPLC): HPLC separates oligos based on differences in their chemical properties, such as size, charge, and hydrophobicity. It provides high purity and is suitable for demanding applications such as oligonucleotide therapeutics, diagnostic assays, and structural studies.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4)&nbsp;&nbsp;Polyacrylamide Gel Electrophoresis (PAGE): PAGE separates oligos based on their size by electrophoresis through a polyacrylamide gel matrix. It is often used for preparative purification of longer oligos (&gt; 30 nucleotides) and is suitable for applications like sequencing, hybridization, and site-directed mutagenesis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5)&nbsp;&nbsp;Reverse-phase Chromatography (RPC): RPC separates oligos based on differences in their hydrophobicity. It is particularly useful for purifying modified oligos and is suitable for applications requiring high purity, such as RNA interference studies and antisense oligonucleotide therapeutics.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6)&nbsp;&nbsp;Capillary Electrophoresis (CE): CE separates oligos based on differences in their charge-to-size ratio by electrophoresis through a capillary tube. It offers high resolution and is suitable for both analytical and preparative purification of oligos for various applications, including genomics, diagnostics, and gene synthesis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Each purification method has its own advantages and limitations, and the choice of method depends on factors such as the required purity, yield, length of oligos, and specific application requirements.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe’s experienced technical team has established the optimized production process and strict quality control standards. We specialize in&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F204.html\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">custom DNA oligo synthesis services&nbsp;\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">&nbsp;\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">of various specifications and purities, supporting diverse needs such as PCR, cloning, site-directed mutagenesis, molecular diagnostics, etc.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","Oligo Synthesis—Oligo Purification Methods","Oligos can be purified using various methods, each with its own advantages and suitability for different applications: desalting, SPE, HPLC, PAGE, RPC, etc. ","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, oligo purification, HPLC purification","2025-04-28 16:20:50",3,"52,49,46,43,42",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":61,"categoryName":9,"title":62,"imgUrl":63,"content":64,"excerpt":13,"publishDate":65,"seoTitle":66,"seoDesc":67,"seoKeywords":68,"createDate":69,"sort":70,"relatedPostIds":71,"firstServiceProductId":21,"secondServiceProductIds":22,"relatedPost":7,"productService":7,"allSecondProductService":7,"twoSecondProductService":7},42,"What are the functions of Spacer modification?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F04_20260630141404A066.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F04_20260630141411A067.jpg\" title=\"Spacer modification, modified oligo\" alt=\"Spacer modification, modified oligo\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Spacer modifications in&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;involve the introduction of non-nucleotide linkers between nucleotides or at specific positions within the oligo sequence. These spacers serve various functions and confer several advantages in oligonucleotide design and applications. Here are some key functions of spacer modifications:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Enhanced flexibility: Spacer modifications can increase the flexibility of oligonucleotides by introducing flexible linker molecules between nucleotides. This increased flexibility can improve the accessibility of oligos to their target molecules and enhance binding kinetics.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Reduced steric hindrance: Spacers can alleviate steric hindrance between adjacent functional groups or molecules, particularly in complex hybridization reactions involving bulky molecules or secondary structures. By providing additional space between nucleotides, spacers can improve hybridization efficiency and specificity.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Prevention of steric clashes: Spacers can prevent steric clashes between functional groups within the oligonucleotide sequence or between the oligo and other biomolecules. This is particularly important in applications such as molecular beacon probes or aptamer-based sensors, where structural integrity is crucial for proper function.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.Modulation of backbone flexibility: Spacer modifications can alter the backbone flexibility of oligonucleotides, affecting their conformational dynamics and interactions with target molecules. This modulation can influence the stability, specificity, and binding kinetics of oligonucleotide-target complexes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Facilitation of conjugation: Spacers can provide attachment points for conjugation of various functional moieties, such as fluorophores, quenchers, affinity tags, or therapeutic payloads. By introducing spacers at specific positions within the oligonucleotide sequence, conjugation sites can be strategically placed to minimize interference with hybridization or biological activity.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Improvement of nuclease resistance: Certain spacer modifications can enhance the nuclease resistance of oligonucleotides by sterically hindering access of nucleases to the phosphodiester backbone. This can prolong the half-life of oligos in biological environments and improve their stability for therapeutic or diagnostic applications.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Regulation of oligonucleotide properties: Spacer modifications allow for fine-tuning of oligonucleotide properties such as solubility, charge, hydrophobicity, and conformational flexibility. By selecting appropriate spacer molecules and incorporating them at specific positions within the oligo sequence, researchers can tailor oligonucleotide characteristics to suit particular applications.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Overall, spacer modifications play a crucial role in\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">&nbsp;\u003C\u002Fspan>\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotide\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;design and optimization, enabling improved performance and expanded functionality in a wide range of biotechnological and biomedical applications.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe provides customized&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">DNA oligo modification\u002Flabeling services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">,\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\"> including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes\u002Fprimers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">modified primers\u002Fprobes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2025-04-28 00:00:00","Oligo Synthesis—Functions of Spacer Modification","Meta Description: Spacer modifications play a crucial role in oligonucleotide design and optimization, enabling improved performance and expanded functionality in a wide range of biotechnological and biomedical applications.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, Spacer modification, modified oligo","2025-04-28 16:21:35",4,"51,50,49,47,46",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":73,"categoryName":9,"title":74,"imgUrl":75,"content":76,"excerpt":13,"publishDate":77,"seoTitle":78,"seoDesc":79,"seoKeywords":80,"createDate":81,"sort":82,"relatedPostIds":83,"firstServiceProductId":21,"secondServiceProductIds":22,"relatedPost":7,"productService":7,"allSecondProductService":7,"twoSecondProductService":7},43,"What are the functions of amino modification?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F2_20260702141223A200.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F2_20260702141242A201.png\" title=\"oligo modifications, primer synthesis\" alt=\"oligo modifications, primer synthesis\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Amino modifications refer to the chemical alterations that introduce amino functional groups into&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotides\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">. This modification can be achieved by incorporating amino groups onto the nucleobases or the sugar moieties of the oligonucleotide. Amino modifications endow oligonucleotides with specific properties and functionalities, offering numerous advantages for various biological and biotechnological applications.\u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Enhanced Stability: Amino modifications can increase the stability of oligonucleotides, making them more resistant to biological samples, such as extracellular environments, intracellular conditions, or sample processing.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Increased Affinity: Amino modifications can enhance the binding affinity of oligonucleotides to their target molecules, thereby improving efficiency and specificity in target recognition and binding processes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Improved Pharmacokinetic Properties: In nucleic acid therapeutics research, amino modifications can improve the pharmacokinetic properties of oligonucleotides, such as prolonging half-life, enhancing cellular uptake, and reducing immune responses.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.Alteration of Charge Properties: Amino modifications can introduce charged amino groups, thereby altering the overall charge properties of oligonucleotides. This is significant for regulating migration rates and separation characteristics in techniques such as electrophoresis, gel electrophoresis, or ion-exchange chromatography.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Introduction of Functional Groups: Amino modifications can introduce specific functional groups onto oligonucleotides, such as amino acid residues, crosslinkers, or other biologically active moieties, thereby imparting specific biological activity or chemical reactivity.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Drug Delivery and Targeting: Amino modifications can be utilized for targeted delivery of oligonucleotide therapeutics, such as by modulating their affinity for cell surface receptors to achieve targeted delivery and internalization into specific cell types.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In summary, amino modifications provide a powerful tool for customizing the properties and functionalities of&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">&nbsp;in various biological and biotechnological fields. By modulating the type, position, and quantity of amino modifications, precise control over the properties and functions of oligonucleotides can be achieved to meet specific research or application requirements.\u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe provides customized&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">DNA oligo modification\u002Flabeling services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes\u002Fprimers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade&nbsp;\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">modified primers\u002Fprobes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-07-02 00:00:00","Oligo Synthesis—Functions of Amino Modification"," Amino modifications provide a powerful tool for customizing the properties and functionalities of oligonucleotides in various biological and biotechnological fields. By modulating the type, position, and quantity of amino modifications, precise control over the properties and functions of oligonucleotides can be achieved to meet specific research or application requirements.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, Amino modification, modified oligo","2025-04-28 16:28:47",5,"53,50,49,47,42",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":21,"title":85,"excerpt":86,"content":87,"seoTitle":88,"seoDesc":89,"seoKeywords":90,"sort":91,"createDate":92,"secondColor":93,"showOrderPic":5,"homeState":34,"homePic":94,"homeTitle":95,"homeDesc":96,"homeSort":91,"orderDesc":97,"orderUrl":98,"email":99,"phone":100,"onlineFormUrl":98,"serviceFeatureList":7,"productServiceSubList":7},"DNA Oligo Synthesis","GenCefe offers high-quality DNA oligo synthesis, including standard primers, modified oligos, qPCR\u002FNGS primers and probes, with reliable performance for diverse research needs.","\u003Cp style=\"line-height: 1.75em;\">\u003Cspan style=\"font-family: Montserrat;\">\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>GenCefe provides high-quality DNA \u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FproductsAndservices\u002F2-DNA_Oligo_Synthesis.html\" style=\"text-decoration: underline; color: rgb(0, 112, 192);\">\u003Cspan style=\"color: rgb(0, 112, 192);\">\u003Cstrong>oligo synthesis\u003C\u002Fstrong>\u003C\u002Fspan>\u003C\u002Fa> and modification services, including standard DNA primers, modified DNA oligos, qPCR primers and probes, and NGS primers and probes. Our DNA oligo synthesis services are supported by standardized production workflows and strict quality control to ensure high consistency, accuracy, and reliable performance across research and in vitro applications.\u003C\u002Fp>\u003Cp style=\"line-height: 1.75em;\">\u003Cspan style=\"font-family: Montserrat;\">\u003C\u002Fspan>\u003C\u002Fp>","DNA Synthesis | Fast Delivery & Reliable Quality-GenCefe","GenCefe provides DNA oligo synthesis services, including standard primers, modified oligos, and qPCR primers and probes for research and IVD applications.","DNA synthesis, DNA oligo synthesis, Custom DNA synthesis, Custom DNA oligo synthesis, DNA oligo, Primer synthesis, Probe synthesis",77,"2024-10-24 15:41:04","#185192","https:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2025\u002F04\u002F20\u002FDNA_蓝_20250420143200A003.png","DNA Synthesis","High-quality primers and probes for research and industrial use","Get high-quality DNA oligos with reliable performance, flexible customization, and efficient delivery—submit your sequence to start your project with GenCefe.","https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM","oligo@gencefebio.com","+1 408-828-0438",[102,108,112,117,121,125,129,134,138],{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":103,"parentId":21,"title":104,"subTitle":105,"excerpt":106,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},62,"Custom DNA Oligo Synthesis","Custom DNA Oligo Synthesis with Flexible Specifications and Reliable Quality","GenCefe provides high-quality oligonucleotide synthesis and modification services, including standard primers, qPCR primers and probes, NGS primers, etc. ","服务",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":109,"parentId":21,"title":110,"subTitle":13,"excerpt":111,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},63,"DNA Oligo Modifications","GenCefe provides comprehensive modifications, including biotin, digoxigenin, fluorescence, phosphorylation, phosphorothioates, amino Linkers, etc.",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":113,"parentId":21,"title":114,"subTitle":115,"excerpt":116,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},64,"qPCR Primers & Probes","Custom qPCR primers and probes with flexible specifications and reliable quality.","GenCefe Biotech has established an advanced oligo synthesis and modification technology platform with an experienced technical team to provide customized qPCR primers and probes.",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":118,"parentId":21,"title":119,"subTitle":13,"excerpt":120,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},65,"NGS Primers & Probes","GenCefe Biotech offers NGS adapters, blockers, capture probes, and multiplex PCR primers using advanced oligo synthesis and modification technology.",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":122,"parentId":21,"title":123,"subTitle":13,"excerpt":124,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},66,"FISH Probes","GenCefe Biotech offers custom FISH probe design and synthesis with various fluorescent labels (Cy3, Cy5, FAM), HPLC purification, and expert technical support.",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":126,"parentId":21,"title":127,"subTitle":13,"excerpt":128,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},67,"STR Primers"," GenCefe offers high-quality fluorescently labeled STR primers for forensic applications, with optimized dyes, purification processes, and strict quality standards.",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":130,"parentId":21,"title":131,"subTitle":132,"excerpt":133,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":34,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},68,"Circular DNA","Custom single-stranded circular DNA synthesis tailored to your research needs. ","GenCefe Biotech has successfully developed single-stranded circular DNA (CssDNA) synthesis technology, providing researchers in gene expression, gene therapy, and vaccine development with a powerful new tool. ",{"createBy":7,"createTime":7,"updateBy":7,"updateTime":7,"remark":7,"id":135,"parentId":21,"title":136,"subTitle":13,"excerpt":137,"content":7,"seoTitle":7,"seoDesc":7,"seoKeywords":7,"createDate":7,"orderDesc":7,"orderUrl":7,"email":7,"phone":7,"onlineFormUrl":7,"sort":7,"hotState":5,"newState":5,"type":107,"customerTestimonials":7,"productCatalog":7,"relatedTitles":7,"productService":7,"customerTestimonialsList":7,"relatedTitlesList":7,"serviceFeatureSubList":7,"productCatalogList":7},70,"PCR Assay Design and Development","GenCefe Biotech combines 10+ years oligo synthesis experience with bioinformatics and AI to offer one-step PCR assay design, synthesis, and verification 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