[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"ApplicationsList":3,"BlogDetail39Info":39,"BasicInfo":177,"ProductServiceList":188},{"msg":4,"code":5,"data":6},"操作成功",0,[7,20,24,28,32,35],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":9,"title":10,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":11,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":12,"productService":8},null,9,"Oligonucleotide Drug Synthesis",90,[13,16],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":9,"orderNum":8,"ancestors":8,"id":14,"title":15,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":11,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},19,"Oligonucleotide Synthesis and Modifications",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":9,"orderNum":8,"ancestors":8,"id":17,"title":18,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":19,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},20,"Oligonucleotide biological evaluation platform",50,{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":21,"title":22,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":23,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},14,"Gene&Genome Editing",80,{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":25,"title":26,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":27,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},15,"Synthetic Biology",70,{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":29,"title":30,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":31,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},16,"Antibody Drug Discovery",60,{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":33,"title":34,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":19,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},17,"IVD Raw Material",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"parentName":8,"parentId":5,"orderNum":8,"ancestors":8,"id":36,"title":37,"excerpt":8,"content":8,"publishDate":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"productServiceId":8,"sort":38,"homeState":8,"homePic":8,"homeTitle":8,"homeDesc":8,"homeSort":8,"applicationsSubList":8,"productService":8},18,"Vaccine and Novel Therapy",40,{"msg":4,"code":5,"data":40},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":41,"categoryName":42,"title":43,"imgUrl":44,"content":45,"excerpt":46,"publishDate":47,"seoTitle":48,"seoDesc":49,"seoKeywords":50,"createDate":51,"sort":52,"relatedPostIds":53,"firstServiceProductId":54,"secondServiceProductIds":55,"relatedPost":56,"productService":117,"allSecondProductService":134,"twoSecondProductService":174},39,"Primers & Probes","What are the applications of Oligo in next-generation sequencing (NGS)?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F01_20260630134319A060.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F01_20260630134326A061.jpg\" title=\"probe synthesis, oligo modifications\" alt=\"probe synthesis, oligo modifications\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">In next-generation sequencing (NGS), \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"font-family: Montserrat; text-decoration: underline;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">oligos\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\"> \u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> (short for oligonucleotides) have various applications, including:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1)  Targeted Sequencing: Oligos are used to design primers for selective amplification of specific DNA or RNA sequences, enabling the study of particular genes, genomic regions, or transcripts.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2)  Library Preparation: Oligos are employed to label DNA or RNA fragments during library preparation processes, facilitating library construction and enrichment.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3)  Polymerase Chain Reaction (PCR): Oligos serve as primers in PCR to amplify target DNA segments, playing a crucial role in DNA replication and amplification.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4)  RNA Capture and Sequencing: Oligos are utilized to capture RNA, such as reverse transcribing RNA and using specific oligos for library enrichment, enabling RNA sequencing analysis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5)  Molecular Barcoding: Oligos can function as molecular barcodes, tagging samples for differentiation in high-throughput sequencing, and aiding in sample tracking and data analysis.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6)  Single Cell Sequencing: In single-cell sequencing, oligos are used to label RNA or DNA of individual cells, facilitating identification and differentiation during subsequent sequencing.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7)  SNP Genotyping: Oligos are used for the detection and genotyping of single nucleotide polymorphisms (SNPs), applicable in genetic disease research, population genetics, among other fields.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8)  DNA\u002FRNA Hybridization: Oligos are employed in hybridization experiments to bind with target DNA or RNA sequences, useful for detection, separation, and quantification of specific sequences.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9)  DNA\u002FRNA Aptamers: Oligos are used for screening and constructing DNA or RNA aptamers, high-selectivity molecular probes capable of binding to specific molecules or proteins.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10)  Epigenetic Analysis: Oligos are utilized for the enrichment and detection of methylation or histone modification sites, aiding in the study of epigenetic changes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\"> \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech’s professional technical team utilizes our advanced \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" style=\"font-family: Montserrat; text-decoration: underline;\">\u003Cspan style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">oligo synthesis and modification\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\"> \u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> technology platform to provide adapters for NGS library construction, blockers and capture probes for hybridization capture, and primers for multiplex PCR. We are committed to supporting your NGS experiments with \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">high-quality primers and probes \u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","","2024-03-29 00:00:00","Oligo Synthesis—Oligo applications in next-generation sequencing (NGS)","In next-generation sequencing (NGS), oligos (short for oligonucleotides) have various applications, including: Targeted Sequencing, Library Preparation, RNA Capture and Sequencing, Molecular Barcoding, etc.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, NGS primer, NGS probe","2025-04-28 16:18:12",1,"53,52,51,50,49",2,"62,65",[57,70,82,95,106],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":58,"categoryName":42,"title":59,"imgUrl":60,"content":61,"excerpt":46,"publishDate":62,"seoTitle":63,"seoDesc":64,"seoKeywords":65,"createDate":66,"sort":67,"relatedPostIds":68,"firstServiceProductId":54,"secondServiceProductIds":69,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},49,"What are the commonly used fluorescent modification groups?","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630142611A080.jpg","\u003Cp>\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F11_20260630142618A081.jpg\" title=\"fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification\" alt=\"fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification\" width=\"100%\"\u002F>\u003C\u002Fp>\u003Cp>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">Oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> (oligos) are frequently modified with various fluorescent groups to enable detection, imaging, and analysis of nucleic acids. Some common fluorescent modifications used in oligos include:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Fluorescein (FAM): FAM is a widely used fluorescent dye that emits green fluorescence when excited by light of appropriate wavelength. It is commonly employed in applications such as real-time PCR, DNA sequencing, and fluorescence in situ hybridization (FISH).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Tetramethylrhodamine (TAMRA): TAMRA is a red fluorescent dye often used as a quencher in oligonucleotide probes. It can quench the fluorescence of nearby fluorophores via fluorescence resonance energy transfer (FRET), making it useful for probe-based assays such as qPCR and molecular beacon assays.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Cyanine Dyes (Cy3, Cy5, Cy5.5, Cy7): Cyanine dyes are a family of fluorescent dyes with varying emission wavelengths. Cy3 emits green fluorescence, Cy5 emits red fluorescence, and Cy5.5 and Cy7 emit near-infrared fluorescence. They are commonly used in multiplexed assays, microarray experiments, and fluorescence imaging techniques.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.Alexa Fluor Dyes: Alexa Fluor dyes are a series of highly fluorescent dyes with a wide range of emission wavelengths. They are characterized by their photostability and brightness, making them suitable for various fluorescence-based applications such as immunofluorescence staining, flow cytometry, and microscopy.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Rhodamine Dyes (ROX, ROX): Rhodamine dyes emit red-orange fluorescence and are often used as reference dyes in real-time PCR and DNA sequencing reactions. They serve as internal standards for normalization of fluorescence signals, aiding in accurate quantification of target nucleic acids.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.BODIPY Dyes: BODIPY dyes are a family of fluorescent dyes with high quantum yields and excellent photostability. They are used in various applications including fluorescence labeling, imaging, and detection of nucleic acids and proteins.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Quasar Dyes: Quasar dyes are a series of proprietary fluorescent dyes developed by Biosearch Technologies. They offer excellent brightness and photostability, making them suitable for applications such as SNP genotyping, FRET assays, and fluorescence in situ hybridization (FISH).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">These are just a few examples of the many fluorescent modifications available for \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligonucleotides\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\"> The choice of fluorescent dye depends on factors such as emission wavelength, compatibility with detection systems, and specific requirements of the experiment or assay.\u003Cbr\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe provides customized\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\"> \u003C\u002Fspan>\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">DNA oligo modification\u002Flabeling services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes\u002Fprimers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">modified primers\u002Fprobes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-07-23 00:00:00","Oligo Synthesis—Fluorescent Modifications","Oligos are frequently modified with various fluorescent groups to enable detection, imaging, and analysis of nucleic acids. Some common fluorescent modifications used in oligos include: FAM, TAMRA, Cyanine Dyes, etc. ","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, fluorescent modification, fluorescent labeling, FAM modification, TAMRA modification, Quasar570 modification, Cy3 Cy5 modification","2025-04-28 16:35:59",11,"43,42,41,40,39","62,63",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":19,"categoryName":42,"title":71,"imgUrl":72,"content":73,"excerpt":46,"publishDate":74,"seoTitle":75,"seoDesc":76,"seoKeywords":77,"createDate":78,"sort":79,"relatedPostIds":80,"firstServiceProductId":54,"secondServiceProductIds":81,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},"Tips for primer design","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F12_20260630142720A082.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F12_20260630142727A083.jpg\" title=\"primer synthesis, primer design\" alt=\"primer synthesis, primer design\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Sequence Specificity: Ensure that the primer sequences are specific to the target region of interest. Use bioinformatics tools to check for potential off-target binding sites and minimize the risk of non-specific amplification. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Melting Temperature (Tm): Aim for similar Tm values between the forward and reverse primers to promote balanced amplification. Tm can be estimated using online calculators based on primer sequence and experimental conditions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.GC Content: The GC content of primers should ideally be around 50-60% to ensure stable hybridization and efficient amplification. Avoid stretches of consecutive Gs or Cs, as they can lead to primer self-annealing or hairpin formation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.Primer Length: Primers are typically 18-25 nucleotides long. Longer primers may provide increased specificity but can also increase the likelihood of primer-dimer formation. Shorter primers may lack specificity. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Avoid Sequence Repeats: Check for repetitive sequences, homopolymeric stretches, or sequence motifs prone to secondary structure formation, as they can interfere with primer annealing and PCR amplification. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Primer Secondary Structure: Avoid regions in the primer sequences that can form stable secondary structures, such as hairpins or self-dimers, as they can hinder primer annealing and affect PCR efficiency. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Avoid Sequence Variability: Be mindful of sequence variations (e.g., SNPs, indels) within the primer binding sites, especially in applications such as SNP genotyping or mutation detection. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8.Primer 3' End Stability: Ensure that the 3' ends of the primers are free of secondary structures or mismatches to facilitate efficient primer annealing and extension during PCR. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9.In Silico Analysis: Utilize bioinformatics tools and software for primer design and evaluation. These tools can predict primer specificity, Tm, secondary structures, and other parameters to optimize primer design. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10.Experimental Validation: Validate primer performance experimentally using PCR, qPCR, or other relevant assays. Optimize PCR conditions (e.g., annealing temperature, Mg2+ concentration) as needed to achieve robust amplification and minimize non-specific products. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">By following these tips, you can design primers that are specific, efficient, and suitable for a wide range of PCR-based applications.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe provides customized \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002Fadmin\u002F%E5%81%9A%E8%BF%87%E7%9A%84%E6%96%87%E4%BB%B6\u002FOligonucleotide%20synthesis%20and%20application.pptx\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">DNA oligo modification\u002Flabeling services\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes\u002Fprimers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F23.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">modified primers\u002Fprobes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-07-25 00:00:00","Oligo Synthesis—Tips for Primer Design","Factors that need to be considered when designing primers include sequence specificity, TM value, GC content, primer length, secondary structure, etc. By following these tips, you can design primers that are specific, efficient, and suitable for a wide range of PCR-based applications.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, primer design","2025-04-28 16:36:57",12,"44,43,42,41,40","62,70",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":83,"categoryName":42,"title":84,"imgUrl":85,"content":86,"excerpt":46,"publishDate":87,"seoTitle":88,"seoDesc":89,"seoKeywords":90,"createDate":91,"sort":92,"relatedPostIds":93,"firstServiceProductId":54,"secondServiceProductIds":94,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},51,"Tips for qPCR probe design ","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F5_20260702141500A207.png","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F07\u002F02\u002F5_20260702141514A208.png\" title=\"qPCR probe design, qPCR probe\" alt=\"qPCR probe design, qPCR probe\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003C\u002Fspan>\u003Cspan style=\"font-family: Montserrat;\">1.Sequence Specificity: Ensure that the probe sequence is specific to the target region of interest. Utilize bioinformatics tools to verify probe specificity by checking against genomic databases for potential off-target binding sites. \u003C\u002Fspan>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Avoid Primer-Probe Interactions: Check for potential interactions between the probe and primers, as well as between different probes if using multiplex qPCR. Ensure that the probe sequence does not overlap with primer binding sites to prevent interference with primer annealing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Probe Length: qPCR probes are typically 20-30 nucleotides long. Longer probes may provide increased specificity but can also decrease sensitivity due to reduced binding efficiency. Shorter probes may be more sensitive but could compromise specificity. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.GC Content: Aim for a GC content of around 40-60% for qPCR probes to ensure stable hybridization. Avoid stretches of consecutive Gs or Cs, as they can lead to probe self-annealing or secondary structure formation. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Melting Temperature (Tm): Calculate the Tm of the probe using software tools based on sequence composition and experimental conditions. Aim for a Tm that is 5-10°C higher than the annealing temperature of the primers to ensure probe binding specificity. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Fluorescent Reporter and Quencher: Select an appropriate fluorescent reporter dye and quencher for the qPCR probe. Common choices include FAM (reporter) and TAMRA (quencher) for hydrolysis probes, or HEX (reporter) and BHQ-1 (quencher) for dual-labeled probes. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Position of Reporter and Quencher: Place the reporter dye at the 5' end and the quencher at the 3' end of the probe. This configuration allows for efficient fluorescence signal generation upon probe cleavage during qPCR amplification. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8.Avoid Secondary Structures: Ensure that the probe sequence does not contain regions prone to secondary structure formation, such as hairpins or self-dimers. These structures can interfere with probe hybridization and affect qPCR efficiency. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9.Experimental Validation: Validate probe performance experimentally using qPCR assays. Optimize qPCR conditions (e.g., annealing temperature, primer and probe concentrations) to achieve robust amplification and reliable quantification of target nucleic acids. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10.Multiplexing Considerations: If performing multiplex qPCR with multiple probes targeting different amplicons, ensure that probes have compatible Tm values and minimal cross-reactivity to enable accurate quantification of multiple targets in a single reaction.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">By following these tips, you can design \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F209.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">qPCR probes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> that are specific, sensitive, and suitable for accurate quantification of target nucleic acids in qPCR assays.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech has established an advanced \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligo synthesis and modification\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> technology platform with an experienced technical team to provide customized \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F209.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">qPCR primers and probes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">. We are committed to assisting your research and diagnostic applications with high accuracy and reliability of \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F209.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">qPCR primers and probes\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-07-30 00:00:00","Oligo Synthesis—Tips for qPCR Probe Design","Factors that need to be considered when designing qPCR probes include sequence specificity, TM value, avoid primer-probe interactions, primer length, fluorescent reporter and quencher, etc. By following these tips, you can design qPCR probes that are specific, sensitive, and suitable for accurate quantification of target nucleic acids in qPCR assays","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, probe design, qPCR probe design, qPCR probe","2025-04-28 16:38:05",13,"46,45,44,43,42","64,70",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":96,"categoryName":42,"title":97,"imgUrl":98,"content":99,"excerpt":46,"publishDate":100,"seoTitle":101,"seoDesc":102,"seoKeywords":103,"createDate":104,"sort":21,"relatedPostIds":105,"firstServiceProductId":54,"secondServiceProductIds":55,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},52,"Tips for NGS capture probe design","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F14_20260630142922A086.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F14_20260630142929A087.jpg\" title=\"probe design, NGS capture probe design, NGS probe\" alt=\"probe design, NGS capture probe design, NGS probe\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Designing \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">capture probes for next-generation sequencing (NGS)\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> involves careful consideration of various factors to ensure efficient target enrichment and sequencing. Here are some tips for NGS capture probe design: \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Target Selection: Clearly define the genomic regions or target sequences of interest for capture. Consider factors such as gene panels, exons, regulatory elements, or specific genomic regions relevant to the research or clinical application. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Probe Length: Design capture probes with lengths typically ranging from 60 to 120 nucleotides. Longer probes may provide increased specificity, while shorter probes may offer better coverage of GC-rich regions or improve capture efficiency. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Probe Density: Optimize the density of capture probes across the target regions to ensure uniform coverage. Balance the number of probes to achieve sufficient capture efficiency without excessive redundancy or cost. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.GC Content: Aim for an average GC content of around 40-60% for capture probes to ensure stable hybridization with target sequences. Adjust probe GC content as needed to accommodate regions with variable GC content. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Tm Uniformity: Ensure uniform melting temperatures (Tm) across capture probes to facilitate consistent hybridization conditions during capture. Aim for similar Tm values to minimize variations in capture efficiency across target regions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Avoid Repeat Regions: Exclude repetitive elements and low-complexity regions from probe design to minimize non-specific binding and improve capture specificity. Utilize bioinformatics tools to mask repetitive sequences and design probes in unique genomic regions. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Proximity to SNPs and Indels: Avoid designing capture probes that overlap with single nucleotide polymorphisms (SNPs) or insertion\u002Fdeletion (indel) variants to prevent interference with hybridization efficiency and sequencing accuracy. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8.Probe Design Algorithms: Utilize bioinformatics software tools specifically designed for capture probe design. These tools consider parameters such as probe specificity, secondary structure, and off-target binding to optimize probe sequences for efficient target enrichment. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9.Consideration of Structural Variants: If targeting regions with known structural variants (SVs), design capture probes to encompass breakpoints or flanking sequences to ensure accurate detection and characterization of SVs during sequencing. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10.Experimental Validation: Validate capture probe performance experimentally using NGS assays. Evaluate capture efficiency, coverage uniformity, and specificity using control samples or reference materials to ensure reliable and reproducible target enrichment. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">By following these tips, you can design capture probes that effectively enrich target sequences for\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\"> \u003C\u002Fspan>\u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">NGS applications\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\">, enabling accurate and comprehensive analysis of genomic regions of interest. \u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech’s professional technical team utilizes our advanced \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligo synthesis and modification technology\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> platform to provide adapters for NGS library construction, blockers and capture probes for hybridization capture, and primers for multiplex PCR. We are committed to supporting your \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">NGS\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> experiments with high-quality primers and probes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-08-01 00:00:00","Oligo Synthesis—Tips for NGS capture probe design","Factors that need to be considered when designing NGS capture probes include target selection, probe length, probe density, GC content, Tm uniformity etc. By following these tips, you can design capture probes that effectively enrich target sequences for NGS applications, enabling accurate and comprehensive analysis of genomic regions of interest.","oligo synthesis, probe synthesis, oligo modifications, primer synthesis, probe design, NGS capture probe design, NGS probe","2025-04-28 16:39:01","48,47,46,45,44",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":107,"categoryName":42,"title":108,"imgUrl":109,"content":110,"excerpt":46,"publishDate":111,"seoTitle":112,"seoDesc":113,"seoKeywords":114,"createDate":115,"sort":25,"relatedPostIds":116,"firstServiceProductId":54,"secondServiceProductIds":81,"relatedPost":8,"productService":8,"allSecondProductService":8,"twoSecondProductService":8},53,"Tips for multiplex PCR primer design","http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F15_20260630143013A088.jpg","\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">\u003Cimg src=\"http:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2026\u002F06\u002F30\u002F15_20260630143019A089.jpg\" title=\"probe design, multiplex PCR primer design, multiplex PCR primer\" alt=\"probe design, multiplex PCR primer design, multiplex PCR primer\" width=\"100%\"\u002F>\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">Designing primers for multiplex PCR requires careful consideration of several factors to ensure specific amplification of multiple targets in a single reaction. Here are some tips for multiplex PCR primer design:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">1.Sequence Specificity: Ensure that each primer pair targets a unique and specific region within the genome or target DNA. Utilize bioinformatics tools to verify primer specificity by checking against genomic databases for potential off-target binding sites.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">2.Avoid Primer-Dimer Formation: Design primers with minimal complementarity to each other to prevent primer-dimer formation, which can lead to non-specific amplification and reduced PCR efficiency. Ensure that the melting temperatures (Tm) of primer pairs are compatible to minimize primer-primer interactions.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">3.Similar Tm Values: Aim for similar Tm values among primer pairs to promote balanced amplification of all targets. Adjust primer lengths and sequences as needed to achieve uniform Tm values across primer pairs.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">4.GC Content: Aim for a GC content of around 50% for each primer to ensure stable primer annealing and efficient amplification. Avoid stretches of consecutive Gs or Cs, as they can lead to secondary structure formation and primer-primer interactions.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">5.Primer Length: Design primers with lengths typically ranging from 18 to 25 nucleotides. Longer primers may provide increased specificity but can also increase the likelihood of primer-dimer formation. Shorter primers may lack specificity.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">6.Proximity to Target Regions: Position primers close to the target regions of interest to ensure efficient amplification. Avoid designing primers across exon-intron boundaries or regions prone to sequence variation to minimize amplification biases.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">7.Amplification Efficiency: Test each primer pair individually in singleplex PCR reactions to determine their amplification efficiency and specificity. Optimize PCR conditions (e.g., annealing temperature, Mg2+ concentration) for each primer pair if necessary.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">8.Labeling and Detection: Consider incorporating different labels (e.g., fluorescent dyes, unique molecular identifiers) into primer sequences for multiplex detection and downstream analysis. Ensure compatibility with the detection method (e.g., gel electrophoresis, real-time PCR).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">9.Consideration of Amplicon Size: Design primers to generate amplicons of similar size to facilitate uniform amplification and detection. Adjust primer positions or amplicon lengths if necessary to achieve comparable amplicon sizes for all targets.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">10.Experimental Validation: Validate multiplex PCR primer sets experimentally using control samples or reference materials. Evaluate amplification specificity, efficiency, and reproducibility across multiple targets to ensure reliable and accurate results.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">By following these tips, you can design multiplex PCR primer sets that enable specific and efficient amplification of multiple targets in a single reaction, facilitating high-throughput and comprehensive analysis of complex samples.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"font-family: Montserrat;\">GenCefe Biotech’s professional technical team utilizes our advanced \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F11.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">oligo synthesis and modification technology\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> platform to provide adapters for NGS library construction, blockers and capture probes for hybridization capture, and primers for multiplex PCR. We are committed to supporting your \u003C\u002Fspan>\u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FOligoSynthesis\u002F213.html\" target=\"_blank\" style=\"text-decoration: underline; font-family: Montserrat; color: rgb(79, 129, 189);\">\u003Cspan style=\"font-family: Montserrat; color: rgb(79, 129, 189);\">NGS\u003C\u002Fspan>\u003C\u002Fa>\u003Cspan style=\"font-family: Montserrat;\"> experiments with high-quality primers and probes.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr\u002F>\u003C\u002Fp>","2024-08-06 00:00:00","Oligo Synthesis—Tips for multiplex PCR primer design"," Factors that need to be considered when designing multiplex PCR primers include sequence specificity, avoid primer-dimer formation, similar Tm values, GC content, primer length, etc. By following these tips, you can design multiplex PCR primer sets that enable specific and efficient amplification of multiple targets in a single reaction, facilitating high-throughput and comprehensive analysis of complex samples."," oligo synthesis, probe synthesis, oligo modifications, primer synthesis, probe design, multiplex PCR primer design, multiplex PCR primer","2025-04-28 16:58:36","52,50,48,45,43",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":54,"title":118,"excerpt":119,"content":120,"seoTitle":121,"seoDesc":122,"seoKeywords":123,"sort":124,"createDate":125,"secondColor":126,"showOrderPic":5,"homeState":52,"homePic":127,"homeTitle":128,"homeDesc":129,"homeSort":124,"orderDesc":130,"orderUrl":131,"email":132,"phone":133,"onlineFormUrl":131,"serviceFeatureList":8,"productServiceSubList":8},"DNA Oligo Synthesis","GenCefe offers high-quality DNA oligo synthesis, including standard primers, modified oligos, qPCR\u002FNGS primers and probes, with reliable performance for diverse research needs.","\u003Cp style=\"line-height: 1.75em;\">\u003Cspan style=\"font-family: Montserrat;\">\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>GenCefe provides high-quality DNA \u003Ca href=\"https:\u002F\u002Fwww.gencefebio.com\u002FproductsAndservices\u002F2-DNA_Oligo_Synthesis.html\" style=\"text-decoration: underline; color: rgb(0, 112, 192);\">\u003Cspan style=\"color: rgb(0, 112, 192);\">\u003Cstrong>oligo synthesis\u003C\u002Fstrong>\u003C\u002Fspan>\u003C\u002Fa> and modification services, including standard DNA primers, modified DNA oligos, qPCR primers and probes, and NGS primers and probes. Our DNA oligo synthesis services are supported by standardized production workflows and strict quality control to ensure high consistency, accuracy, and reliable performance across research and in vitro applications.\u003C\u002Fp>\u003Cp style=\"line-height: 1.75em;\">\u003Cspan style=\"font-family: Montserrat;\">\u003C\u002Fspan>\u003C\u002Fp>","DNA Synthesis | Fast Delivery & Reliable Quality-GenCefe","GenCefe provides DNA oligo synthesis services, including standard primers, modified oligos, and qPCR primers and probes for research and IVD applications.","DNA synthesis, DNA oligo synthesis, Custom DNA synthesis, Custom DNA oligo synthesis, DNA oligo, Primer synthesis, Probe synthesis",77,"2024-10-24 15:41:04","#185192","https:\u002F\u002Fmanage.gencefebio.com\u002Fprofile\u002Fupload\u002F2025\u002F04\u002F20\u002FDNA_蓝_20250420143200A003.png","DNA Synthesis","High-quality primers and probes for research and industrial use","Get high-quality DNA oligos with reliable performance, flexible customization, and efficient delivery—submit your sequence to start your project with GenCefe.","https:\u002F\u002Fforms.zohopublic.com\u002Fgencefebiotech\u002Fform\u002FRNAsynthesisQuoteForm\u002Fformperma\u002FIYj3YS_MNOP-_4ozkpMtj2ONDpoS9eqBGWF3bBvb2xM","oligo@gencefebio.com","+1 408-828-0438",[135,141,145,150,154,158,162,167,170],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":136,"parentId":54,"title":137,"subTitle":138,"excerpt":139,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},62,"Custom DNA Oligo Synthesis","Custom DNA Oligo Synthesis with Flexible Specifications and Reliable Quality","GenCefe provides high-quality oligonucleotide 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technology.",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":155,"parentId":54,"title":156,"subTitle":46,"excerpt":157,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},66,"FISH Probes","GenCefe Biotech offers custom FISH probe design and synthesis with various fluorescent labels (Cy3, Cy5, FAM), HPLC purification, and expert technical 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",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":27,"parentId":54,"title":168,"subTitle":46,"excerpt":169,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},"PCR Assay Design and Development","GenCefe Biotech combines 10+ years oligo synthesis experience with bioinformatics and AI to offer one-step PCR assay design, synthesis, and verification services.",{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":171,"parentId":54,"title":172,"subTitle":46,"excerpt":173,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},71,"DNA Oligo QC","GenCefe Biotech has a strict ISO9001 & ISO13485-certified QC system for oligos, offering standard and customized QC for research and IVD applications.",[175,176],{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":136,"parentId":54,"title":137,"subTitle":138,"excerpt":139,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"createBy":8,"createTime":8,"updateBy":8,"updateTime":8,"remark":8,"id":151,"parentId":54,"title":152,"subTitle":46,"excerpt":153,"content":8,"seoTitle":8,"seoDesc":8,"seoKeywords":8,"createDate":8,"orderDesc":8,"orderUrl":8,"email":8,"phone":8,"onlineFormUrl":8,"sort":8,"hotState":5,"newState":5,"type":140,"customerTestimonials":8,"productCatalog":8,"relatedTitles":8,"productService":8,"customerTestimonialsList":8,"relatedTitlesList":8,"serviceFeatureSubList":8,"productCatalogList":8},{"msg":4,"code":5,"data":178},{"companyName":179,"shortCompanyName":180,"title":180,"subTitle":181,"siteUrl":182,"siteDescription":183,"siteKeywords":184,"beiAnInfo":185,"email":186,"phone":133,"address":187},"GENCEFE Biotech (Wuxi, China) ","GenCefe","Your Reliable Partner of Oligo, Gene, Peptide, Protein ","https:\u002F\u002Fwww.gencefebio.com\u002F","GenCefe specializes in DNA, RNA, gene, peptide, and protein synthesis. 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