circRNA

GenCefe Biotech has successfully developed a chemical synthesis technology for circRNA, complementing our in vitro transcription (IVT) circRNA platform. This enables us to provide tailored circRNA solutions with varying lengths and purity to meet your diverse research needs.


Why Choose GenCefe circRNA Synthesis Services?

     ●      Dual-Platform Technology: With both chemical synthesis and IVT platforms, we can produce circRNA of different lengths, modifications, and applications, ensuring flexibility for your research.

     ●      Chemical Synthesis Platform: Specializing in short circRNA segments (≤100 nt), we offer high-purity, high-yield, and fast-turnaround synthesis, ideal for miRNA sponges and functional fragment screening.

     ●      IVT Platform: Designed for efficient synthesis of long circRNA (≥200 nt), suitable for functional studies and drug development.

     ●      Expert Team: With extensive experience in circular oligonucleotide synthesis, our team provides professional technical support and fully customized services to meet your specific requirements.


Case Study:

Synthesize 25 nt RNA oligo, and connect the 5' phosphate end of the single-stranded RNA to the 3' hydroxyl end through ligase to form a circular structure.

Left: LC-MS during the cyclization reaction

Right: LC-MS after the cyclization reaction

Fig. 1 The target molecular weight before and after cyclization differs by 17.6, which is consistent with the characteristics of linearized RNA cyclization. During the cyclization of linearized RNA, an additional phosphodiester  bond is formed, and a water molecule is removed, resulting in a decrease in molecular weight of 18.

Fig. 2 Verification of RNA cyclization using linear RNA digestion enzyme.  Linear and circular RNA fragments of the same size were treated with linear  digestion enzyme. The linear RNA was digested, while the circular RNA showed  no significant change.

1: Marker

2: Linear RNA

3: Linear RNA treated with linear digestion enzyme (digested)

4: Circular RNA

5: Circular RNA treated with linear digestion enzyme (undigested) 


Service Specifications:

Length

Turnaround Time

Order

circRNA Synthesis

≤60 nt

7+ days

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61-100 nt

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IVT circRNA

>200 nt

2+ weeks

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