Circular DNA

GenCefe Biotech has successfully developed single-stranded circular DNA (CssDNA) synthesis technology, providing researchers in gene expression, gene therapy, and vaccine development with a powerful new tool.

Why Choose GenCefe CssDNA Synthesis Service?

● Enhanced Stability: The circular DNA structure prevents degradation in cells, ensuring long-term stable expression.

● High Transfection Efficiency: Compared to linear DNA, CssDNA offers superior transfection efficiency, making it particularly suitable for gene therapy and DNA vaccine development.

● Versatile Applications: Whether for gene expression vectors, virology studies, nanotechnology, or gene editing, our CssDNA provides reliable and stable support.

● Custom Solutions: From sequence design to synthesis, we offer fully customized services to meet your specific research needs.

Case Study:

Synthesize ~20 nt DNA oligo, and connect the 5' phosphate end of the single-stranded DNA to the 3' hydroxyl end through ligase to form a circular structure.

Left: LC-MS during the cyclization reaction

Right: LC-MS after the cyclization reaction

Fig. 1 The target molecular weight before and after cyclization differs by 18.8, which is consistent with the characteristics of linearized DNA cyclization. During the cyclization of linearized DNA, an additional phosphodiester bond is formed, and a water molecule is removed, resulting in a decrease in molecular weight of 18.

Fig. 2 Verification of DNA cyclization using linear DNA digestion enzyme. Linear and circular DNA fragments of the same size were treated with linear digestion enzyme. The linear DNA was digested, while the circular DNA showed no significant change.

1: Marker

2: Linear DNA

3: Linear DNA treated with linear digestion enzyme (digested)

4: Circular DNA

5: Circular DNA treated with linear digestion enzyme (undigested)

Service Specifications:

	Length	Turnaround Time	Order
CssDNA Synthesis	≤60 nt	7+ days	Get a Quote
CssDNA Synthesis	61-100 nt	Get a Quote	Get a Quote

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EMAIL	PHONE	ONLINE FORM
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