Applications-Oligonucleotide Synthesis and Modifications

Oligonucleotide Synthesis and Modifications

GenCefe Biotech has established a complete oligonucleotide synthesis and modification platform, with advanced synthesizers of different fluxes to meet the synthesis requirements of different specifications. Professional R&D and technical teams and mature synthesis, purification, and modification methods, to provide high-quality oligonucleotides for nucleic acid drug researchers. We provide siRNA, antisense oligonucleotide, miRNA, nucleic acid aptamer, and sgRNA synthesis, and can also customize the synthesis of various nucleic acid sequences. According to the needs of customers, we can design and synthesize RNA oligos of different lengths, different forms, and with various modifications, and we can also synthesize RNA sequences designed by customers. The synthesis specifications are flexible, and OD, mg, gram, and hectogram levels can be selected for synthesis. All products are purified by HPLC, implement strict QC standards, and meet ISO9001 quality management system certification standards.

Oligonucleotide Synthesis

Type	Product Name	Size	Purification	Turnaround Time (days)
siRNA	siRNA	OD to hectogram	HPLC	Starting from 4 days
	Modified siRNA	Get a Quote		Get a Quote
	siRNA controls	1 OD		In stock
	siRNA Guaranteed Package siRNA oligos 3 pairs (3×2 OD) or 4 pairs (4×4 OD) Negative control siRNA oligos 1×1 OD FAM-labeled negative control siRNA oligos 1×1 OD Positive control siRNA oligos 1×1 OD		HPLC	Starting from 5 days
miRNA (in vitro)	microRNA mimic	Starting from 2 OD	HPLC	Starting from 4 days
	microRNA inhibitor
	microRNA mimics N.C.
	microRNA inhibitor N.C.
miRNA (in vivo)	microRNA agomir	Starting from 2 OD	HPLC	Starting from 7 days
	microRNA antagomir
	microRNA agomir N.C.
	microRNA antagomir N.C.
Antisense Oligonucleotides (ASOs)	ASOs	μg to gram	HPLC	Get a Quote
	Modified ASOs
Nucleic acid aptamer	Aptamer	μg to gram	HPLC	Get a Quote
	Modified Aptamer
sgRNA	Chemically synthesized sgRNA	Starting from 2 OD	HPLC	Starting from 7 days

Chemical Modification and Fluorescent Labeling Services

Nucleic Acid Type	Chemical Modification and Fluorescent Labeling Services
siRNA	Sugar Modification: 2'-Ome, 2'-F, etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: Chol (cholesterol), Biotin, Thiol, NH2, etc. Fluorescent labeling: Cy3, Cy5, etc. Customized chemical modification and fluorescent labeling proposal design
Antisense (ASO)	Sugar Modification: 2'-OMe, 2'-MOE, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: Chol (cholesterol), etc.
Aptamer	Sugar Modification: 2'-OMe, 2'-NH2, 2'-F, LNA, etc. Base Modification: methylated cytosine (5-Me-dC), etc. Backbone Modification: PS (phosphorothioate) modification, etc. End modification: 3'-inverted thymidine, Chol (cholesterol), Biotin, Thiol, NH2, fluorescent dyes, etc.
Others	Custom chemical modification and fluorescent labeling services

Delivery System Customization and Conjugation Services

Delivery System	Features	Services
GalNAc (N-acetylgalactosamine)	GalNAc is the targeting ligand of the sialic acid receptor (ASGPR), which is mainly expressed on the surface of hepatic parenchymal cells. The conjugate of nucleic acid and GalNAc enters the cell through endocytosis to function.	Custom GalNAc Synthesis GalNAc-siRNA Synthesis GalNAc-ASO Synthesis GalNAc-miRNA Synthesis Customized design and synthesis of GalNAc delivery proposal
Peptide	Peptides have better membrane permeability and strong resistance to degradation. Therefore, conjugating oligonucleotides with various biological functions to peptides can not only improve the biological activity of oligonucleotides but also enhance their cell permeability.	Peptide Synthesis (drug peptides, custom peptides, RGD cyclic peptides, etc.) Peptide-siRNA Conjugation Peptide-ASO Conjugation Peptide-DNA Conjugation RGD-siRNA Conjugation
Lipid	LNP not only prevents degradation of delivered oligonucleotides and prolongs circulation time, but also facilitates oligonucleotide uptake, enhances intracellular transport, and facilitates endosomal escape.	LNP-siRNA Conjugation LNP-ASO Conjugation
PEG (polyethylene glycol)	The half-life of PEG-modified ASO in the body is significantly prolonged, and PEG can reduce the excretion rate of oligonucleotides and promote the uptake of nucleic acid drugs by corresponding cells.	PEG-Aptamer Conjugation PEG-siRNA Conjugation PEG-ASO Conjugation
Others	/	Custom delivery proposal design and synthesis

QC Standards and Custom QC Services

GenCefe Biotech is committed to providing high-quality oligonucleotide products and services; we perform HPLC detection and provide COA files for all of our oligonucleotide products by default. There’s also a variety of customized QC service items to meet the specific needs of users.

Customized QC items (additional fee will be applied)

Molecular weight determination of oligonucleotides
Sequence confirmation of oligonucleotides
Impurity identification of oligonucleotides
Melting temperature (Tm) of oligonucleotide
IEX-HPLC Purity of Oligonucleotides
RP-HPLC Purity of Oligonucleotides
SEC-HPLC Purity of Oligonucleotides
Endotoxin content of oligonucleotides

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EMAIL	PHONE	ONLINE FORM
[email protected]	+1 408-828-0438	ONLINE QUOTE SUBMISSION

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