qPCR (quantitative polymerase chain reaction), also called real-time PCR, is a molecular biology technique used to quickly and sensitively measure the amount of a specific gene in a DNA or RNA sample. Primers and probes are one of the key factors affecting the success of qPCR and the reliability of its results.

GenCefe Biotech has established an advanced oligo synthesis and modification technology platform with an experienced technical team to provide customized qPCR primers and probes. We are committed to assisting your research and diagnostic applications with high accuracy and reliability of qPCR primers and probes.

Service Advantages:

• ISO9001 certified, GMP-like clean room, strict control of pollution sources, avoiding NTC interference

• Advanced production technology ensures product accuracy, high purity, and stable fluorescent labeling

• Standardized operation and strict quality control to ensure batch-to-batch stability of primers/probes

qPCR Primers & Probes Case Studies:

1.Comparison of Probe Fluorescence Intensity

	Under the same experimental conditions, after 120 minutes of incubation, the probe of GenCefe has a higher fluorescence signal intensity, which is about 1.5 times higher than that of Company SG. This shows that the probe synthesized by GenCefe has higher sensitivity

2.FAM probe multi-component enzyme digestion verification

	The probes synthesized by GenCefe has a high fluorescence intensity, indicating that the probes have high sensitivity. Compared with the probes synthesized by Company S, the probe synthesized by GenCefe shows better stability.

3.TaqMan Probe Comparison

TaqMan Probe (Company GS)	TaqMan Probe (GenCefe)	Compared with the probes provided by Company SG, the GenCefe probes showed better Ct values and fluorescence intensity, and the detection results in different channels were stable.
Detection result of GenCefe TaqMan Probes in different channels

4.Dual-Quenching Probe

Dual-Quenching Probe (Company TF)	Dual Quenching Probe (GenCefe)
Compared with similar probes from TF, the GenCefe dual-quenching probe shows better Ct value and fluorescence intensity, with the fluorescence intensity increased by >100%.

qPCR Primers & Probes Service Specifications:

Please download and fill out the  oligo order form   and send it via email to  [email protected] . Please note the minimum order size of custom oligo synthesis is $50/order.

Modification/Labeling/Dual-Labeling: view the full list