An important application of CRISPR/Cas technology is to precisely insert or replace DNA sequences at specific genomic sites through the homology directed repair (HDR). ssDNA (single-stranded DNA)/ssODN (single-stranded oligonucleotides) is an ideal choice for HDR templates, with significant advantages such as low cytotoxicity, high specific knockout efficiency, and significantly reduced off-target effects.

Based on GenCefe Biotech's well-established gene synthesis platform and rich experience in molecular biology, we provide high-quality single-stranded DNA (ssDNA) synthesis services. The ssDNA we synthesize can reach several thousand bases, with high purity and no double-strand residues, and can be used in cell-based and in vivo experiments.

Service advantages:

• Quality assurance: verified by Sanger sequencing before delivery to ensure accurate sequences and no double-strand residues
• Low cytotoxicity: High purity, no dsDNA contamination, suitable for cell-based and in vivo experiments
• Difficult sequences: rich synthesis experience, can successfully deliver forward/reverse repeats and other difficult sequences

Case Study:

1. Sanger sequencing
ssDNA sequencing verification: the sequencing results shows that the forward sequencing verification sequence is correct, and there is no signal in the reverse primer sequencing, proving that there is no double-stranded residue in the ssDNA.

2. Enzyme Digestion Verification

Lane 1: ssDNA sample Lane M: marker

Service Specifications:

   * HPLC purification is available upon request for preclinical grade ssDNA.