CRISPR-Cas9 system is the most popular genome-editing tool for genome modification with high efficiency, simple operation, and low cytotoxicity.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas9) system exerts genome editing effect through cleaving DNA double strands using RNA-guided endonuclease (sgRNA). Double-strand breaks (DSB) were repaired by one of two major mechanisms that occur in almost all cell types and organisms: homology-directed repair (HDR) or non-homologous end joining (NHEJ), accompanied by insertions, deletions, or replacements into the genome. As a powerful tool, the CRISPR/Cas9 system has provided tremendous convenience for basic researches and may pave the path to treat genetic diseases and cancers. GeneCefe can offer reliable CRISPR sgRNA and Cas9 vector construct and sgRNA library genome screening service to accelerate your research.

Advantages of the CRISPR-Cas9 system

• Flexibility edits genes/genomes across a wide range of species, such as human cell lines, plants, yeast, mice, etc.
• Multiple genes can be edited simultaneously with various sgRNAs.
• Ease to design/use: An exchange of a 20-bp spacer sequence in the gRNA can easily reprogram Cas9 to target a different DNA site.

Workflow of Genome Editing