As CRISPR research advances, the need for scalable sgRNA libraries has grown. From focused custom panels targeting specific pathways to genome-wide libraries encompassing thousands of genes, scalable libraries empower high-throughput functional genomics and drug discovery.

Custom Panels for Targeted Research

Custom sgRNA panels allow researchers to focus on defined gene sets:

●  Pathway analysis: Target specific signaling or metabolic pathways.

●  Disease-relevant genes: Concentrate on oncogenes, immune regulators, or neurological targets.

●  Experimental flexibility: Adjust library size, coverage, and controls to suit research needs.

Genome-Wide Libraries for Discovery

Genome-wide libraries provide comprehensive coverage of all coding or non-coding regions:

●  High-throughput screening: Identify essential genes, drug targets, or resistance pathways.

●  Functional genomics: Systematically interrogate gene function across the entire genome.

●  Phenotypic diversity: Analyze multiple cellular responses simultaneously for deeper insights.

Key Considerations

●  Representation: Ensure uniform distribution of sgRNAs for accurate results.

●  Specificity: Minimize off-target effects with careful sequence design.

●  Scalability: Support for different library sizes, from hundreds to tens of thousands of sgRNAs.

GenCefe Biotech Solutions

GenCefe Biotech provides custom and genome-wide sgRNA library synthesis with high fidelity and uniformity. Our scalable solutions enable both targeted research and large-scale screening, supporting drug discovery, functional genomics, and translational applications.