RNA stability is a critical factor in the success of CRISPR-mediated genome editing. Chemical modifications to sgRNA and pegRNA can enhance their stability, reduce degradation, and improve overall editing efficiency.

Common RNA Modifications

● 2’-O-methyl and 3’ phosphorothioate linkages: Protect RNA ends from exonuclease degradation.

●  Locked nucleic acids (LNAs): Enhance binding affinity to target sequences.

●  Fluorescent or biotin labels: Facilitate tracking and experimental monitoring.

These modifications increase the intracellular half-life of sgRNAs and pegRNAs, enabling more efficient targeting and higher editing rates.

Applications and Benefits

●  Enhanced genome editing: Stable RNAs maintain activity long enough to complete precise edits.

●  Reduced off-target effects: Modifications improve specificity and reduce unintended cleavage.

●  Support for therapeutic applications: Improved RNA stability is essential for ex vivo and in vivo gene editing therapies.

GenCefe Biotech Solutions

GenCefe Biotech provides custom synthesis of chemically modified sgRNAs and pegRNAs, tailored for stability, efficiency, and experimental needs. Our services enable researchers to optimize genome editing outcomes for basic research, therapeutic development, and advanced CRISPR applications.